The NOP receptor involvement in both withdrawal- and CCk-8-induced contracture responses of guinea pig isolated ileum after acute activation of k-opioid receptor

Pietro Marini, Luca Romanelli, Daniela Valeri, Maria Grazia Cascio, Paolo Tucci, Pacifico Valeri, Maura Palmery

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In isolated guinea-pig ileum (GPI), the ¿-opioid acute withdrawal response is under the control of several neuronal signaling systems, including the µ-opioid, the A1-adenosine and the CB1 receptors, which are involved in the inhibitory control of the ¿-withdrawal response. After ¿-opioid system stimulation, indirect activation of µ-opioid, A1-adenosine and CB1 systems is prevented by the peptide cholecystokinin-8 (CCk-8). In the present study, we have investigated whether the NOP system is also involved in the regulation of the acute ¿-withdrawal response. Interestingly, we found that in GPI preparation, the NOP system is not indirectly activated by the ¿-opioid receptor stimulation, but instead this system is able by itself to directly regulate the acute ¿-withdrawal response. Specifically, our results clearly highlight first the existence of an endogenous tone of the NOP system in GPI, and second that it behaves as a functional anti-opioid system. We also found that, the NOP receptor system is involved in the regulation of the CCk-8-induced contracture intensity, only when in the presence of the ¿-opioid receptor stimulation. This effect seems to be regulated by an activation threshold mechanism. In conclusion, the NOP system could act as neuromodulatory system, whose action is strictly related to the modulation of both excitatory and inhibitory neurotransmitters released in GPI enteric nervous system.
Original languageEnglish
Pages (from-to)418-426
Number of pages9
JournalPeptides
Volume38
Issue number2
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Opioid Receptors
Contracture
Ileum
Opioid Analgesics
Guinea Pigs
Chemical activation
Adenosine
Enteric Nervous System
Cannabinoid Receptor CB1
Purinergic P1 Receptors
Neurology
Neurotransmitter Agents
Modulation
cholecystokinin 8
Peptides

Keywords

  • OFQ/N
  • UFP-101
  • NOP receptor
  • κ-Opioid receptor
  • CCk-8
  • GPI
  • opioid withdrawal

Cite this

The NOP receptor involvement in both withdrawal- and CCk-8-induced contracture responses of guinea pig isolated ileum after acute activation of k-opioid receptor. / Marini, Pietro; Romanelli, Luca; Valeri, Daniela ; Cascio, Maria Grazia; Tucci, Paolo; Valeri, Pacifico; Palmery, Maura.

In: Peptides, Vol. 38, No. 2, 12.2012, p. 418-426.

Research output: Contribution to journalArticle

Marini, Pietro ; Romanelli, Luca ; Valeri, Daniela ; Cascio, Maria Grazia ; Tucci, Paolo ; Valeri, Pacifico ; Palmery, Maura. / The NOP receptor involvement in both withdrawal- and CCk-8-induced contracture responses of guinea pig isolated ileum after acute activation of k-opioid receptor. In: Peptides. 2012 ; Vol. 38, No. 2. pp. 418-426.
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AU - Palmery, Maura

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AB - In isolated guinea-pig ileum (GPI), the ¿-opioid acute withdrawal response is under the control of several neuronal signaling systems, including the µ-opioid, the A1-adenosine and the CB1 receptors, which are involved in the inhibitory control of the ¿-withdrawal response. After ¿-opioid system stimulation, indirect activation of µ-opioid, A1-adenosine and CB1 systems is prevented by the peptide cholecystokinin-8 (CCk-8). In the present study, we have investigated whether the NOP system is also involved in the regulation of the acute ¿-withdrawal response. Interestingly, we found that in GPI preparation, the NOP system is not indirectly activated by the ¿-opioid receptor stimulation, but instead this system is able by itself to directly regulate the acute ¿-withdrawal response. Specifically, our results clearly highlight first the existence of an endogenous tone of the NOP system in GPI, and second that it behaves as a functional anti-opioid system. We also found that, the NOP receptor system is involved in the regulation of the CCk-8-induced contracture intensity, only when in the presence of the ¿-opioid receptor stimulation. This effect seems to be regulated by an activation threshold mechanism. In conclusion, the NOP system could act as neuromodulatory system, whose action is strictly related to the modulation of both excitatory and inhibitory neurotransmitters released in GPI enteric nervous system.

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