Abstract
A melatonin-related receptor was cloned from an ovine genomic library, The sequenced gene has a similar structure to that of the melatonin receptor gene family and consists of two exons separated by an intron of approximately 3 kb, Exon 1 and exon 2 of the ovine melatonin-related receptor encode a protein of 575 amino acids which is 73.8% homologous to the human melatonin-related receptor and shows 40.9% homology with the ovine Mel(1a) melatonin receptor. COS-7 cells transiently expressing ovine melatonin-related receptors did not bind 2-[I-125]iodomelatonin or H-3-melatonin. Reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization studies revealed expression of the ovine melatonin-related receptor in the hypothalamus, pituitary, retina and retinal pigment epithelium, Furthermore, expression of the ovine melatonin-related receptor is shown to be coincident with Mel(1a) and 2-[I-125]iodomelatonin binding in the pituitary and serotonin N-acetyl transferase (arylalkylamine N-acetyl transferase, AANAT) expression in the retina. Expression patterns and similarity with the melatonin receptor gene family suggest a role for this novel G protein-coupled receptor in control and regulation of endocrine function and retinal physiology.
| Original language | English |
|---|---|
| Pages (from-to) | 651-661 |
| Number of pages | 11 |
| Journal | Journal of Neuroendocrinology |
| Volume | 10 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 1998 |
Keywords
- melatonin-related receptors
- melatonin receptors
- hypothalamus
- pituitary
- retina
- N-acetyl transferase
- ovine
- 2-<I-125>IODOMELATONIN BINDING
- expression cloning
- pars-tuberalis
- messenger-RNA
- protein
- identification
- localization
- cells
Cite this
The ovine melatonin-related receptor : Cloning and Preliminary Distribution and Binding Studies. / Drew, Janice; Barrett, Perry; Williams, Lynda; Conway, S ; Morgan, Peter John.
In: Journal of Neuroendocrinology, Vol. 10, No. 9, 09.1998, p. 651-661.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ovine melatonin-related receptor
T2 - Cloning and Preliminary Distribution and Binding Studies
AU - Drew, Janice
AU - Barrett, Perry
AU - Williams, Lynda
AU - Conway, S
AU - Morgan, Peter John
PY - 1998/9
Y1 - 1998/9
N2 - A melatonin-related receptor was cloned from an ovine genomic library, The sequenced gene has a similar structure to that of the melatonin receptor gene family and consists of two exons separated by an intron of approximately 3 kb, Exon 1 and exon 2 of the ovine melatonin-related receptor encode a protein of 575 amino acids which is 73.8% homologous to the human melatonin-related receptor and shows 40.9% homology with the ovine Mel(1a) melatonin receptor. COS-7 cells transiently expressing ovine melatonin-related receptors did not bind 2-[I-125]iodomelatonin or H-3-melatonin. Reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization studies revealed expression of the ovine melatonin-related receptor in the hypothalamus, pituitary, retina and retinal pigment epithelium, Furthermore, expression of the ovine melatonin-related receptor is shown to be coincident with Mel(1a) and 2-[I-125]iodomelatonin binding in the pituitary and serotonin N-acetyl transferase (arylalkylamine N-acetyl transferase, AANAT) expression in the retina. Expression patterns and similarity with the melatonin receptor gene family suggest a role for this novel G protein-coupled receptor in control and regulation of endocrine function and retinal physiology.
AB - A melatonin-related receptor was cloned from an ovine genomic library, The sequenced gene has a similar structure to that of the melatonin receptor gene family and consists of two exons separated by an intron of approximately 3 kb, Exon 1 and exon 2 of the ovine melatonin-related receptor encode a protein of 575 amino acids which is 73.8% homologous to the human melatonin-related receptor and shows 40.9% homology with the ovine Mel(1a) melatonin receptor. COS-7 cells transiently expressing ovine melatonin-related receptors did not bind 2-[I-125]iodomelatonin or H-3-melatonin. Reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization studies revealed expression of the ovine melatonin-related receptor in the hypothalamus, pituitary, retina and retinal pigment epithelium, Furthermore, expression of the ovine melatonin-related receptor is shown to be coincident with Mel(1a) and 2-[I-125]iodomelatonin binding in the pituitary and serotonin N-acetyl transferase (arylalkylamine N-acetyl transferase, AANAT) expression in the retina. Expression patterns and similarity with the melatonin receptor gene family suggest a role for this novel G protein-coupled receptor in control and regulation of endocrine function and retinal physiology.
KW - melatonin-related receptors
KW - melatonin receptors
KW - hypothalamus
KW - pituitary
KW - retina
KW - N-acetyl transferase
KW - ovine
KW - 2-<I-125>IODOMELATONIN BINDING
KW - expression cloning
KW - pars-tuberalis
KW - messenger-RNA
KW - protein
KW - identification
KW - localization
KW - cells
U2 - 10.1046/j.1365-2826.1998.00229.x
DO - 10.1046/j.1365-2826.1998.00229.x
M3 - Article
VL - 10
SP - 651
EP - 661
JO - Journal of Neuroendocrinology
JF - Journal of Neuroendocrinology
SN - 0953-8194
IS - 9
ER -