The pattern recognition receptors dectin-2, mincle, and FcRγ impact the dynamics of phagocytosis of Candida, Saccharomyces, Malassezia, and Mucor species

Mohammed Haider, Ivy Dambuza, Patawee Asamaphan, Mark Stappers, Delyth Reid, Sho Yamasaki, Gordon D. Brown, Neil A. R. Gow (Corresponding Author), Lars P. Erwig (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

Phagocytosis is a receptor-mediated process critical to innate immune clearance of pathogens. It proceeds in a regulated sequence of stages: (a) migration of phagocytes towards pathogens, (b) recognition of PAMPs and binding through PRRs, (c) engulfment and internalisation into phagosomes, (d) phagosome maturation, and (e) killing of pathogen or host cells. However, little is known about the role that individual receptors play in these discrete stages in the recognition of fungal cells. In a previous study, we found that dectin-2 deficiency impacted some but not all stages of macrophage-mediated phagocytosis of Candida glabrata. Because the C-type lectin receptor dectin-2 critically requires coupling to the FcRγ chain for signalling, we hypothesised that this coupling may be important for regulating phagocytosis of fungal cargo. We therefore examined how deficiency in FcRγ itself or two receptors to which it couples (dectin-2 and mincle) impacts phagocytosis of six fungal organisms representing three different fungal taxa. Our data show that deficiency in these proteins impairs murine bone marrow-derived macrophage migration, engulfment, and phagosome maturation, but not macrophage survival. Therefore, FcRγ engagement with selective C-type lectin receptors (CLRs) critically affects the spatio-temporal dynamics of fungal phagocytosis.

Original languageEnglish
Article number0220867
JournalPloS ONE
Volume14
Issue number8
DOIs
Publication statusPublished - 8 Aug 2019

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Malassezia
Mucor
Pattern Recognition Receptors
Saccharomyces
Candida
Macrophages
Pathogens
phagocytosis
Phagocytosis
C-Type Lectins
phagosomes
Phagosomes
receptors
macrophages
lectins
pathogens
Bone
Candida glabrata
Protein Deficiency
protein deficiencies

Keywords

  • C-TYPE LECTIN
  • HOST-DEFENSE
  • PATHOGEN INTERACTIONS
  • ACTIVATING RECEPTOR
  • ALBICANS
  • MATURATION
  • PHAGOSOMES
  • RESPONSES
  • INSIGHTS
  • FAMILY

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The pattern recognition receptors dectin-2, mincle, and FcRγ impact the dynamics of phagocytosis of Candida, Saccharomyces, Malassezia, and Mucor species. / Haider, Mohammed; Dambuza, Ivy; Asamaphan, Patawee; Stappers, Mark; Reid, Delyth; Yamasaki, Sho; Brown, Gordon D.; Gow, Neil A. R. (Corresponding Author); Erwig, Lars P. (Corresponding Author).

In: PloS ONE, Vol. 14, No. 8, 0220867, 08.08.2019.

Research output: Contribution to journalArticle

Haider, Mohammed ; Dambuza, Ivy ; Asamaphan, Patawee ; Stappers, Mark ; Reid, Delyth ; Yamasaki, Sho ; Brown, Gordon D. ; Gow, Neil A. R. ; Erwig, Lars P. / The pattern recognition receptors dectin-2, mincle, and FcRγ impact the dynamics of phagocytosis of Candida, Saccharomyces, Malassezia, and Mucor species. In: PloS ONE. 2019 ; Vol. 14, No. 8.
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abstract = "Phagocytosis is a receptor-mediated process critical to innate immune clearance of pathogens. It proceeds in a regulated sequence of stages: (a) migration of phagocytes towards pathogens, (b) recognition of PAMPs and binding through PRRs, (c) engulfment and internalisation into phagosomes, (d) phagosome maturation, and (e) killing of pathogen or host cells. However, little is known about the role that individual receptors play in these discrete stages in the recognition of fungal cells. In a previous study, we found that dectin-2 deficiency impacted some but not all stages of macrophage-mediated phagocytosis of Candida glabrata. Because the C-type lectin receptor dectin-2 critically requires coupling to the FcRγ chain for signalling, we hypothesised that this coupling may be important for regulating phagocytosis of fungal cargo. We therefore examined how deficiency in FcRγ itself or two receptors to which it couples (dectin-2 and mincle) impacts phagocytosis of six fungal organisms representing three different fungal taxa. Our data show that deficiency in these proteins impairs murine bone marrow-derived macrophage migration, engulfment, and phagosome maturation, but not macrophage survival. Therefore, FcRγ engagement with selective C-type lectin receptors (CLRs) critically affects the spatio-temporal dynamics of fungal phagocytosis.",
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