The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature

E Lorenzen, K Einer-Jensen, J S Rasmussen, T E Kjaer, B Collet, C Secombes, N Lorenzen

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

DNA vaccines encoding the viral glycoproteins of viral haemorrhagic septicaemia virus (VHSV) and infectious haematopoietic necrosis virus (IHNV) have proved highly efficient in rainbow trout (Oncorhynchus mykiss) under experimental conditions. Non-specific as well as specific immune mechanisms seem to be activated. Temperature is an important external parameter affecting the immune response in fish. The present study aimed at determining the effectiveness of a DNA vaccine against VHS at different temperatures. Rainbow trout fingerlings acclimated at 5 °C, 10 °C or 15 °C, were given an intramuscular injection of 1 µg purified plasmid DNA and challenged with virulent VHSV 8 or 36–40 days later. The vaccine protected the fish well at all three temperatures, but the involvement of innate and adaptive mechanisms differed: at low temperature, non-specific protection lasted longer and at 36 dpv fish kept at 5 °C had no detectable response of neutralizing antibodies while 67% of the fish kept at 15 °C had seroconverted. Induction of Mx as measured in liver samples was delayed at 5 °C with no detectable response 7 dpv whereas fish maintained at 10 °C had significantly elevated levels of Mx3-transcripts at that time point. Immunohistochemical studies of the injection site of vaccinated fish also showed a clear effect of temperature: in fish maintained at 15 °C the vhsG-protein appeared earlier on the surface of transfected myocytes and the inflammatory response clearing away these myocytes arose earlier compared to fish kept at the lower temperatures of 5 and 10 °C.
Original languageEnglish
Pages (from-to)3870-3880
Number of pages11
JournalVaccine
Volume27
Issue number29
Early online date23 Apr 2009
DOIs
Publication statusPublished - 12 Jun 2009

Fingerprint

Rhabdoviridae
DNA Vaccines
recombinant vaccines
Fishes
Temperature
fish
Oncorhynchus mykiss
temperature
Novirhabdovirus
Viral hemorrhagic septicemia virus
myocytes
Muscle Cells
Infectious hematopoietic necrosis virus
injection site
Intramuscular Injections
fingerlings
intramuscular injection
Protein C
Neutralizing Antibodies
neutralizing antibodies

Keywords

  • fish DNA vaccine
  • immunity
  • temperature

Cite this

Lorenzen, E., Einer-Jensen, K., Rasmussen, J. S., Kjaer, T. E., Collet, B., Secombes, C., & Lorenzen, N. (2009). The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature. Vaccine, 27(29), 3870-3880. https://doi.org/10.1016/j.vaccine.2009.04.012

The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature. / Lorenzen, E; Einer-Jensen, K; Rasmussen, J S; Kjaer, T E; Collet, B; Secombes, C; Lorenzen, N.

In: Vaccine, Vol. 27, No. 29, 12.06.2009, p. 3870-3880.

Research output: Contribution to journalArticle

Lorenzen, E, Einer-Jensen, K, Rasmussen, JS, Kjaer, TE, Collet, B, Secombes, C & Lorenzen, N 2009, 'The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature', Vaccine, vol. 27, no. 29, pp. 3870-3880. https://doi.org/10.1016/j.vaccine.2009.04.012
Lorenzen E, Einer-Jensen K, Rasmussen JS, Kjaer TE, Collet B, Secombes C et al. The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature. Vaccine. 2009 Jun 12;27(29):3870-3880. https://doi.org/10.1016/j.vaccine.2009.04.012
Lorenzen, E ; Einer-Jensen, K ; Rasmussen, J S ; Kjaer, T E ; Collet, B ; Secombes, C ; Lorenzen, N. / The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature. In: Vaccine. 2009 ; Vol. 27, No. 29. pp. 3870-3880.
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