The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa

W H Schnippenkoetter, D P Martin, F L Hughes, M Fyvie, J A Willment, D James, M B von Wechmar, E P Rybicki

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The genomic nucleotide sequences of the cloned agroinfectious genomes of three South African mastreviruses obtained from Zea mays, a Setaria sp., and Panicum maximum (designated MSV-Kom, MSV-Set, and PanSV-Kar respectively), were determined. Additionally, their relative infectivities and virulence were analysed in a range of differentially susceptible wheat, maize, and barley genotypes. MSV-Kom produced moderate to severe streak symptoms in all maize genotypes tested, but only moderate to very mild symptoms in the wheat and barley genotypes. MSV-Set infected only the susceptible to tolerant maize genotypes, but was generally more severe in the barley and wheat genotypes than MSV-Kom. PanSV-Kar was incapable of infecting any of the wheat and barley genotypes and only produced very mild symptoms on the three most sensitive maize genotypes. Genomic characteristics in common with related mastreviruses were identified. Phylogenetic analysis indicated that while MSV-Kom was closely related to previously sequenced MSV isolates, MSV-Set and PanSV-Kar represented distinctly novel strains of MSV and PanSV respectively. In the case of MSV-Set, this is the most distantly related MSV strain yet characterised.
Original languageEnglish
Pages (from-to)1075-88
Number of pages14
JournalArchives of Virology
Volume146
Issue number6
Publication statusPublished - 2001

Fingerprint

Geminiviridae
South Africa
Genotype
Zea mays
Hordeum
Triticum
Panicum
Virulence
Genome

Keywords

  • Animals
  • Base Sequence
  • DNA, Intergenic
  • DNA, Viral
  • Geminiviridae
  • Genome, Viral
  • Hemiptera
  • Hordeum
  • Molecular Sequence Data
  • Panicum
  • Phylogeny
  • Plant Diseases
  • South Africa
  • Species Specificity
  • Triticum
  • Virulence
  • Zea mays

Cite this

Schnippenkoetter, W. H., Martin, D. P., Hughes, F. L., Fyvie, M., Willment, J. A., James, D., ... Rybicki, E. P. (2001). The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa. Archives of Virology, 146(6), 1075-88.

The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa. / Schnippenkoetter, W H; Martin, D P; Hughes, F L; Fyvie, M; Willment, J A; James, D; von Wechmar, M B; Rybicki, E P.

In: Archives of Virology, Vol. 146, No. 6, 2001, p. 1075-88.

Research output: Contribution to journalArticle

Schnippenkoetter, WH, Martin, DP, Hughes, FL, Fyvie, M, Willment, JA, James, D, von Wechmar, MB & Rybicki, EP 2001, 'The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa', Archives of Virology, vol. 146, no. 6, pp. 1075-88.
Schnippenkoetter WH, Martin DP, Hughes FL, Fyvie M, Willment JA, James D et al. The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa. Archives of Virology. 2001;146(6):1075-88.
Schnippenkoetter, W H ; Martin, D P ; Hughes, F L ; Fyvie, M ; Willment, J A ; James, D ; von Wechmar, M B ; Rybicki, E P. / The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa. In: Archives of Virology. 2001 ; Vol. 146, No. 6. pp. 1075-88.
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AB - The genomic nucleotide sequences of the cloned agroinfectious genomes of three South African mastreviruses obtained from Zea mays, a Setaria sp., and Panicum maximum (designated MSV-Kom, MSV-Set, and PanSV-Kar respectively), were determined. Additionally, their relative infectivities and virulence were analysed in a range of differentially susceptible wheat, maize, and barley genotypes. MSV-Kom produced moderate to severe streak symptoms in all maize genotypes tested, but only moderate to very mild symptoms in the wheat and barley genotypes. MSV-Set infected only the susceptible to tolerant maize genotypes, but was generally more severe in the barley and wheat genotypes than MSV-Kom. PanSV-Kar was incapable of infecting any of the wheat and barley genotypes and only produced very mild symptoms on the three most sensitive maize genotypes. Genomic characteristics in common with related mastreviruses were identified. Phylogenetic analysis indicated that while MSV-Kom was closely related to previously sequenced MSV isolates, MSV-Set and PanSV-Kar represented distinctly novel strains of MSV and PanSV respectively. In the case of MSV-Set, this is the most distantly related MSV strain yet characterised.

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