The repertoire of killer cell Ig-like receptor and CD94

NKG2A receptors in T cells: clones sharing identical alpha beta TCR rearrangement express highly diverse killer cell Ig-like receptor patterns

M Uhrberg, N M Valiante, Neil Thomas Young, L L Lanier, J H Phillips, P Parham

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Killer cell Ig-like receptor (KIR) and CD94:NKG2A molecules were first defined as human NK cell receptors (NKR), but now are known to be expressed and to function on subpopulations of T cells. Here the repertoires of KIR and CD94:NKG2A expression by T cells from two donors were examined and compared with their previously defined NK cell repertoires. T cell clones generated from peripheral blood of both donors expressed multiple NKR in different combinations and used the range of receptors expressed by NK cells. In both donors alpha beta T cells less frequently expressed the inhibitory receptors CD94:NKG2A and KIR2DL1 than either gamma delta T cells or NK cells. In contrast to NK cells, not all NKR(+) T cells expressed an inhibitory receptor for autologous HLA class I. This lack of specific inhibitory NKR was especially apparent on alpha beta T cells of one donor. Overall, alpha beta T cells exhibited a distinct pattern of NKR expression different from that of gamma delta T and NK cells, which expressed highly similar NKR repertoires. In one donor, analysis of TCR rearrangement revealed a dominant subset of NKR(+) T cells sharing identical TCR alpha- and beta-chains. Remarkably, among 55 T cell clones sharing the same TCR alpha beta rearrangement 18 different KIR phenotypes were seen, suggesting that KIR expression was initiated subsequently to TCR rearrangement.
Original languageEnglish
Pages (from-to)3923-3932
Number of pages10
JournalThe Journal of Immunology
Volume166
Issue number6
Publication statusPublished - 15 Mar 2001

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NK Cell Lectin-Like Receptor Subfamily C
Natural Killer Cell Receptors
Clone Cells
T-Lymphocytes
Natural Killer Cells
Tissue Donors
Somatostatin-Secreting Cells
KIR2DL1 Receptors
Costimulatory and Inhibitory T-Cell Receptors
Blood Donors

Keywords

  • Amino Acid Sequence
  • Antigens, CD
  • Base Sequence
  • Cell Line
  • Clone Cells
  • Flow Cytometry
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Immunoglobulins
  • Killer Cells, Natural
  • Lectins, C-Type
  • Membrane Glycoproteins
  • Molecular Sequence Data
  • NK Cell Lectin-Like Receptor Subfamily C
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Immunologic
  • Receptors, KIR
  • Receptors, KIR2DL1
  • Receptors, Natural Killer Cell
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets

Cite this

The repertoire of killer cell Ig-like receptor and CD94 : NKG2A receptors in T cells: clones sharing identical alpha beta TCR rearrangement express highly diverse killer cell Ig-like receptor patterns. / Uhrberg, M; Valiante, N M; Young, Neil Thomas; Lanier, L L; Phillips, J H; Parham, P.

In: The Journal of Immunology, Vol. 166, No. 6, 15.03.2001, p. 3923-3932.

Research output: Contribution to journalArticle

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abstract = "Killer cell Ig-like receptor (KIR) and CD94:NKG2A molecules were first defined as human NK cell receptors (NKR), but now are known to be expressed and to function on subpopulations of T cells. Here the repertoires of KIR and CD94:NKG2A expression by T cells from two donors were examined and compared with their previously defined NK cell repertoires. T cell clones generated from peripheral blood of both donors expressed multiple NKR in different combinations and used the range of receptors expressed by NK cells. In both donors alpha beta T cells less frequently expressed the inhibitory receptors CD94:NKG2A and KIR2DL1 than either gamma delta T cells or NK cells. In contrast to NK cells, not all NKR(+) T cells expressed an inhibitory receptor for autologous HLA class I. This lack of specific inhibitory NKR was especially apparent on alpha beta T cells of one donor. Overall, alpha beta T cells exhibited a distinct pattern of NKR expression different from that of gamma delta T and NK cells, which expressed highly similar NKR repertoires. In one donor, analysis of TCR rearrangement revealed a dominant subset of NKR(+) T cells sharing identical TCR alpha- and beta-chains. Remarkably, among 55 T cell clones sharing the same TCR alpha beta rearrangement 18 different KIR phenotypes were seen, suggesting that KIR expression was initiated subsequently to TCR rearrangement.",
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T1 - The repertoire of killer cell Ig-like receptor and CD94

T2 - NKG2A receptors in T cells: clones sharing identical alpha beta TCR rearrangement express highly diverse killer cell Ig-like receptor patterns

AU - Uhrberg, M

AU - Valiante, N M

AU - Young, Neil Thomas

AU - Lanier, L L

AU - Phillips, J H

AU - Parham, P

PY - 2001/3/15

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N2 - Killer cell Ig-like receptor (KIR) and CD94:NKG2A molecules were first defined as human NK cell receptors (NKR), but now are known to be expressed and to function on subpopulations of T cells. Here the repertoires of KIR and CD94:NKG2A expression by T cells from two donors were examined and compared with their previously defined NK cell repertoires. T cell clones generated from peripheral blood of both donors expressed multiple NKR in different combinations and used the range of receptors expressed by NK cells. In both donors alpha beta T cells less frequently expressed the inhibitory receptors CD94:NKG2A and KIR2DL1 than either gamma delta T cells or NK cells. In contrast to NK cells, not all NKR(+) T cells expressed an inhibitory receptor for autologous HLA class I. This lack of specific inhibitory NKR was especially apparent on alpha beta T cells of one donor. Overall, alpha beta T cells exhibited a distinct pattern of NKR expression different from that of gamma delta T and NK cells, which expressed highly similar NKR repertoires. In one donor, analysis of TCR rearrangement revealed a dominant subset of NKR(+) T cells sharing identical TCR alpha- and beta-chains. Remarkably, among 55 T cell clones sharing the same TCR alpha beta rearrangement 18 different KIR phenotypes were seen, suggesting that KIR expression was initiated subsequently to TCR rearrangement.

AB - Killer cell Ig-like receptor (KIR) and CD94:NKG2A molecules were first defined as human NK cell receptors (NKR), but now are known to be expressed and to function on subpopulations of T cells. Here the repertoires of KIR and CD94:NKG2A expression by T cells from two donors were examined and compared with their previously defined NK cell repertoires. T cell clones generated from peripheral blood of both donors expressed multiple NKR in different combinations and used the range of receptors expressed by NK cells. In both donors alpha beta T cells less frequently expressed the inhibitory receptors CD94:NKG2A and KIR2DL1 than either gamma delta T cells or NK cells. In contrast to NK cells, not all NKR(+) T cells expressed an inhibitory receptor for autologous HLA class I. This lack of specific inhibitory NKR was especially apparent on alpha beta T cells of one donor. Overall, alpha beta T cells exhibited a distinct pattern of NKR expression different from that of gamma delta T and NK cells, which expressed highly similar NKR repertoires. In one donor, analysis of TCR rearrangement revealed a dominant subset of NKR(+) T cells sharing identical TCR alpha- and beta-chains. Remarkably, among 55 T cell clones sharing the same TCR alpha beta rearrangement 18 different KIR phenotypes were seen, suggesting that KIR expression was initiated subsequently to TCR rearrangement.

KW - Amino Acid Sequence

KW - Antigens, CD

KW - Base Sequence

KW - Cell Line

KW - Clone Cells

KW - Flow Cytometry

KW - Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor

KW - Gene Rearrangement, beta-Chain T-Cell Antigen Receptor

KW - Humans

KW - Immunoglobulins

KW - Killer Cells, Natural

KW - Lectins, C-Type

KW - Membrane Glycoproteins

KW - Molecular Sequence Data

KW - NK Cell Lectin-Like Receptor Subfamily C

KW - NK Cell Lectin-Like Receptor Subfamily D

KW - Receptors, Antigen, T-Cell, alpha-beta

KW - Receptors, Antigen, T-Cell, gamma-delta

KW - Receptors, Immunologic

KW - Receptors, KIR

KW - Receptors, KIR2DL1

KW - Receptors, Natural Killer Cell

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocyte Subsets

M3 - Article

VL - 166

SP - 3923

EP - 3932

JO - The Journal of Immunology

JF - The Journal of Immunology

SN - 0022-1767

IS - 6

ER -