The repertoire of killer cell Ig-like receptor and CD94: NKG2A receptors in T cells: clones sharing identical alpha beta TCR rearrangement express highly diverse killer cell Ig-like receptor patterns

M Uhrberg, N M Valiante, Neil Thomas Young, L L Lanier, J H Phillips, P Parham

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Killer cell Ig-like receptor (KIR) and CD94:NKG2A molecules were first defined as human NK cell receptors (NKR), but now are known to be expressed and to function on subpopulations of T cells. Here the repertoires of KIR and CD94:NKG2A expression by T cells from two donors were examined and compared with their previously defined NK cell repertoires. T cell clones generated from peripheral blood of both donors expressed multiple NKR in different combinations and used the range of receptors expressed by NK cells. In both donors alpha beta T cells less frequently expressed the inhibitory receptors CD94:NKG2A and KIR2DL1 than either gamma delta T cells or NK cells. In contrast to NK cells, not all NKR(+) T cells expressed an inhibitory receptor for autologous HLA class I. This lack of specific inhibitory NKR was especially apparent on alpha beta T cells of one donor. Overall, alpha beta T cells exhibited a distinct pattern of NKR expression different from that of gamma delta T and NK cells, which expressed highly similar NKR repertoires. In one donor, analysis of TCR rearrangement revealed a dominant subset of NKR(+) T cells sharing identical TCR alpha- and beta-chains. Remarkably, among 55 T cell clones sharing the same TCR alpha beta rearrangement 18 different KIR phenotypes were seen, suggesting that KIR expression was initiated subsequently to TCR rearrangement.
Original languageEnglish
Pages (from-to)3923-3932
Number of pages10
JournalThe Journal of Immunology
Volume166
Issue number6
Publication statusPublished - 15 Mar 2001

Keywords

  • Amino Acid Sequence
  • Antigens, CD
  • Base Sequence
  • Cell Line
  • Clone Cells
  • Flow Cytometry
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Immunoglobulins
  • Killer Cells, Natural
  • Lectins, C-Type
  • Membrane Glycoproteins
  • Molecular Sequence Data
  • NK Cell Lectin-Like Receptor Subfamily C
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Immunologic
  • Receptors, KIR
  • Receptors, KIR2DL1
  • Receptors, Natural Killer Cell
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocyte Subsets

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