The role of semiochemicals in host location and non-host avoidance by salmon louse (Lepeophtheirus salmonis) copepodids

R. J. E. Bailey, M. A. Birkett, Anna Ingvarsdottir, Anne Jennifer Mordue, William Mordue, Bridget Anne O'Shea, J. A. Pickett, L. J. Wadhams

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The role and identity of host and non-host chemical cues (semiochemicals) in host location and non-host avoidance for copepodid larvae of sea lice, Lepeophtheirus salmonis, was investigated using Y-tube behavioural bioassays, solid-phase extraction (SPE), and coupled gas chromatography - mass spectrometry (GC-MS). Using artificial seawater conditioned with the preferred salmonid host, Salmo salar, L. salmonis displayed high activation and directional responses in Y-tube assays to salmon-conditioned water (SCW), to an extract of SCW prepared by SPE, and to a vacuum distillate of the SPE extract. Similar responses were observed to two chemicals identified from SCW by coupled GC-MS: isophorone and 6-methyl-5-hepten-2-one. Dose-response studies with isophorone showed that copepodid responses across the range tested were maximised at 0.01 and 0.1 mg center dot mL(-1). A mixture of isophorone and 6-methyl-5-hepten-2-one also induced high activation and directional responses. Semiochemicals were also isolated from the non-host fish, turbot (Scophthalmus maximus (Rafinesque)), by SPE and analysed by GC-MS. Two non-host-specific chemicals were identified as 2-aminoacetophenone and 4-methylquinazoline. When SCW was mixed with either of the non-host chemicals, activation and directional responses to SCW were eliminated in the Y tube.

Original languageEnglish
Pages (from-to)448-456
Number of pages8
JournalCanadian Journal of Fisheries and Aquatic Sciences
Volume63
DOIs
Publication statusPublished - 2006

Keywords

  • ATLANTIC SALMON
  • SEA LOUSE
  • LICE
  • IDENTIFICATION
  • RESPONSES
  • SALINITY
  • BIOLOGY
  • KROYER
  • FARMS
  • LIGHT

Cite this

The role of semiochemicals in host location and non-host avoidance by salmon louse (Lepeophtheirus salmonis) copepodids. / Bailey, R. J. E.; Birkett, M. A.; Ingvarsdottir, Anna; Mordue, Anne Jennifer; Mordue, William; O'Shea, Bridget Anne; Pickett, J. A.; Wadhams, L. J.

In: Canadian Journal of Fisheries and Aquatic Sciences, Vol. 63, 2006, p. 448-456.

Research output: Contribution to journalArticle

Bailey, R. J. E. ; Birkett, M. A. ; Ingvarsdottir, Anna ; Mordue, Anne Jennifer ; Mordue, William ; O'Shea, Bridget Anne ; Pickett, J. A. ; Wadhams, L. J. / The role of semiochemicals in host location and non-host avoidance by salmon louse (Lepeophtheirus salmonis) copepodids. In: Canadian Journal of Fisheries and Aquatic Sciences. 2006 ; Vol. 63. pp. 448-456.
@article{1b2de23ddd69433186e41ba97854009f,
title = "The role of semiochemicals in host location and non-host avoidance by salmon louse (Lepeophtheirus salmonis) copepodids",
abstract = "The role and identity of host and non-host chemical cues (semiochemicals) in host location and non-host avoidance for copepodid larvae of sea lice, Lepeophtheirus salmonis, was investigated using Y-tube behavioural bioassays, solid-phase extraction (SPE), and coupled gas chromatography - mass spectrometry (GC-MS). Using artificial seawater conditioned with the preferred salmonid host, Salmo salar, L. salmonis displayed high activation and directional responses in Y-tube assays to salmon-conditioned water (SCW), to an extract of SCW prepared by SPE, and to a vacuum distillate of the SPE extract. Similar responses were observed to two chemicals identified from SCW by coupled GC-MS: isophorone and 6-methyl-5-hepten-2-one. Dose-response studies with isophorone showed that copepodid responses across the range tested were maximised at 0.01 and 0.1 mg center dot mL(-1). A mixture of isophorone and 6-methyl-5-hepten-2-one also induced high activation and directional responses. Semiochemicals were also isolated from the non-host fish, turbot (Scophthalmus maximus (Rafinesque)), by SPE and analysed by GC-MS. Two non-host-specific chemicals were identified as 2-aminoacetophenone and 4-methylquinazoline. When SCW was mixed with either of the non-host chemicals, activation and directional responses to SCW were eliminated in the Y tube.",
keywords = "ATLANTIC SALMON, SEA LOUSE, LICE, IDENTIFICATION, RESPONSES, SALINITY, BIOLOGY, KROYER, FARMS, LIGHT",
author = "Bailey, {R. J. E.} and Birkett, {M. A.} and Anna Ingvarsdottir and Mordue, {Anne Jennifer} and William Mordue and O'Shea, {Bridget Anne} and Pickett, {J. A.} and Wadhams, {L. J.}",
year = "2006",
doi = "10.1139/f05-231",
language = "English",
volume = "63",
pages = "448--456",
journal = "Canadian Journal of Fisheries and Aquatic Sciences",
issn = "0706-652X",
publisher = "CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS",

}

TY - JOUR

T1 - The role of semiochemicals in host location and non-host avoidance by salmon louse (Lepeophtheirus salmonis) copepodids

AU - Bailey, R. J. E.

AU - Birkett, M. A.

AU - Ingvarsdottir, Anna

AU - Mordue, Anne Jennifer

AU - Mordue, William

AU - O'Shea, Bridget Anne

AU - Pickett, J. A.

AU - Wadhams, L. J.

PY - 2006

Y1 - 2006

N2 - The role and identity of host and non-host chemical cues (semiochemicals) in host location and non-host avoidance for copepodid larvae of sea lice, Lepeophtheirus salmonis, was investigated using Y-tube behavioural bioassays, solid-phase extraction (SPE), and coupled gas chromatography - mass spectrometry (GC-MS). Using artificial seawater conditioned with the preferred salmonid host, Salmo salar, L. salmonis displayed high activation and directional responses in Y-tube assays to salmon-conditioned water (SCW), to an extract of SCW prepared by SPE, and to a vacuum distillate of the SPE extract. Similar responses were observed to two chemicals identified from SCW by coupled GC-MS: isophorone and 6-methyl-5-hepten-2-one. Dose-response studies with isophorone showed that copepodid responses across the range tested were maximised at 0.01 and 0.1 mg center dot mL(-1). A mixture of isophorone and 6-methyl-5-hepten-2-one also induced high activation and directional responses. Semiochemicals were also isolated from the non-host fish, turbot (Scophthalmus maximus (Rafinesque)), by SPE and analysed by GC-MS. Two non-host-specific chemicals were identified as 2-aminoacetophenone and 4-methylquinazoline. When SCW was mixed with either of the non-host chemicals, activation and directional responses to SCW were eliminated in the Y tube.

AB - The role and identity of host and non-host chemical cues (semiochemicals) in host location and non-host avoidance for copepodid larvae of sea lice, Lepeophtheirus salmonis, was investigated using Y-tube behavioural bioassays, solid-phase extraction (SPE), and coupled gas chromatography - mass spectrometry (GC-MS). Using artificial seawater conditioned with the preferred salmonid host, Salmo salar, L. salmonis displayed high activation and directional responses in Y-tube assays to salmon-conditioned water (SCW), to an extract of SCW prepared by SPE, and to a vacuum distillate of the SPE extract. Similar responses were observed to two chemicals identified from SCW by coupled GC-MS: isophorone and 6-methyl-5-hepten-2-one. Dose-response studies with isophorone showed that copepodid responses across the range tested were maximised at 0.01 and 0.1 mg center dot mL(-1). A mixture of isophorone and 6-methyl-5-hepten-2-one also induced high activation and directional responses. Semiochemicals were also isolated from the non-host fish, turbot (Scophthalmus maximus (Rafinesque)), by SPE and analysed by GC-MS. Two non-host-specific chemicals were identified as 2-aminoacetophenone and 4-methylquinazoline. When SCW was mixed with either of the non-host chemicals, activation and directional responses to SCW were eliminated in the Y tube.

KW - ATLANTIC SALMON

KW - SEA LOUSE

KW - LICE

KW - IDENTIFICATION

KW - RESPONSES

KW - SALINITY

KW - BIOLOGY

KW - KROYER

KW - FARMS

KW - LIGHT

U2 - 10.1139/f05-231

DO - 10.1139/f05-231

M3 - Article

VL - 63

SP - 448

EP - 456

JO - Canadian Journal of Fisheries and Aquatic Sciences

JF - Canadian Journal of Fisheries and Aquatic Sciences

SN - 0706-652X

ER -