The use of formalin fixed wax embedded tissue for proteomic analysis

Lynda D. Ralton, Graeme I. Murray

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The potential of proteomic approaches to elucidate disease pathogenesis and biomarker discovery is increasingly being recognised. These studies are usually based on the use of fresh tissue samples. Problems in obtaining and storing fresh frozen samples, especially either for the investigation of rare diseases or for the study of microscopic disease foci, have led to the investigation of the possible use of formalin fixed wax embedded tissue for proteomic biomarker detection Overcoming problems with protein cross-linking associated with formalin fixation of tissues, especially by using heat-mediated retrieval techniques combined with highly sensitive methods for protein separation and identification are now emerging, giving promise to the use of formalin fixed wax embedded tissues for proteomic analysis. Formalin fixed wax embedded tissues, together with their associated clinical and pathological information outcome may provide significant potential opportunities for proteomics research. Such studies of formalin fixed wax embedded tissue will allow access to already acquired clinical tissue samples which can be readily correlated with clinical, pathological and outcome data. It also provides access to rare types of tissue/diseases that would be either difficult to collect prospectively in a timely manner or are unlikely to be available as fresh samples. The purpose of this review is to provide an overview of the issues associated with the use of formalin fixed wax embedded tissues for proteomics.
Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalJournal of Clinical Pathology
Volume64
Issue number4
Early online date1 Feb 2011
DOIs
Publication statusPublished - Apr 2011

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Waxes
Proteomics
Formaldehyde
Rare Diseases
Biomarkers
Tissue Fixation
Proteins
Hot Temperature
Research

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The use of formalin fixed wax embedded tissue for proteomic analysis. / Ralton, Lynda D.; Murray, Graeme I.

In: Journal of Clinical Pathology, Vol. 64, No. 4, 04.2011, p. 297-302.

Research output: Contribution to journalArticle

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