The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation

Sylvie Janssens, Olaf van den Broekm, Ian R. Davenport, Robbert C. Akkers, Fei Liu, Gert Jan C. Veenstr, Stefan Hoppler, Kris Vleminckx, Olivier Destree*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalInternational Journal of Developmental Biology
Issue number1
Publication statusPublished - 2013


  • foxd3
  • lefl/tcf
  • tcf7
  • Xenopus
  • Wnt
  • factor-binding sites
  • beta-catenin
  • differential expression
  • mesoderm development
  • distinct roles
  • transcription
  • genes
  • organizer
  • embryos
  • axis


Dive into the research topics of 'The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation'. Together they form a unique fingerprint.

Cite this