The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation

Sylvie Janssens, Olaf van den Broekm, Ian R. Davenport, Robbert C. Akkers, Fei Liu, Gert Jan C. Veenstr, Stefan Hoppler, Kris Vleminckx, Olivier Destree*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalInternational Journal of Developmental Biology
Volume57
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • foxd3
  • lefl/tcf
  • tcf7
  • Xenopus
  • Wnt
  • factor-binding sites
  • beta-catenin
  • differential expression
  • mesoderm development
  • distinct roles
  • transcription
  • genes
  • organizer
  • embryos
  • axis

Cite this

Janssens, S., van den Broekm, O., Davenport, I. R., Akkers, R. C., Liu, F., Veenstr, G. J. C., ... Destree, O. (2013). The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation. International Journal of Developmental Biology, 57(1), 49-54. https://doi.org/10.1387/ijdb.120191kv

The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation. / Janssens, Sylvie; van den Broekm, Olaf; Davenport, Ian R.; Akkers, Robbert C.; Liu, Fei; Veenstr, Gert Jan C.; Hoppler, Stefan; Vleminckx, Kris; Destree, Olivier.

In: International Journal of Developmental Biology, Vol. 57, No. 1, 2013, p. 49-54.

Research output: Contribution to journalArticle

Janssens, S, van den Broekm, O, Davenport, IR, Akkers, RC, Liu, F, Veenstr, GJC, Hoppler, S, Vleminckx, K & Destree, O 2013, 'The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation', International Journal of Developmental Biology, vol. 57, no. 1, pp. 49-54. https://doi.org/10.1387/ijdb.120191kv
Janssens, Sylvie ; van den Broekm, Olaf ; Davenport, Ian R. ; Akkers, Robbert C. ; Liu, Fei ; Veenstr, Gert Jan C. ; Hoppler, Stefan ; Vleminckx, Kris ; Destree, Olivier. / The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation. In: International Journal of Developmental Biology. 2013 ; Vol. 57, No. 1. pp. 49-54.
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abstract = "TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.",
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author = "Sylvie Janssens and {van den Broekm}, Olaf and Davenport, {Ian R.} and Akkers, {Robbert C.} and Fei Liu and Veenstr, {Gert Jan C.} and Stefan Hoppler and Kris Vleminckx and Olivier Destree",
note = "We thank Dr. B. Gumbiner for providing the rabbit polyclonal antibody against Xenopus b-catenin and S.A. Blythe for sharing the Xenopus ChIP protocol. We thank Tim Deceunink,Thomas Roose and Yvonne Turnbull for animal care taking and Amin Bredan for editing the manuscript. O.v.d.B. and O.D. were supported by the IOP Genomics program (IGE01010), which is subsidized by the Dutch Ministry of Economic Affairs. We acknowledge additional support by the BBSRC (I.R.D. and S.H.) the AICR (F.L. and S.H.), the Netherlands Organization for Scientific Research, Earth and Life Sciences Council (NWO-ALW VIDI 864.03.002, R.C.A. and G.J.C.V.), the Research Foundation-Flanders, the Interuniversity Attraction Poles (IAP7/07), the Belgian Association against Cancer and the Concerted Research Actions (GOA) of Ghent University (S.J. and K.V.)",
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AU - Hoppler, Stefan

AU - Vleminckx, Kris

AU - Destree, Olivier

N1 - We thank Dr. B. Gumbiner for providing the rabbit polyclonal antibody against Xenopus b-catenin and S.A. Blythe for sharing the Xenopus ChIP protocol. We thank Tim Deceunink,Thomas Roose and Yvonne Turnbull for animal care taking and Amin Bredan for editing the manuscript. O.v.d.B. and O.D. were supported by the IOP Genomics program (IGE01010), which is subsidized by the Dutch Ministry of Economic Affairs. We acknowledge additional support by the BBSRC (I.R.D. and S.H.) the AICR (F.L. and S.H.), the Netherlands Organization for Scientific Research, Earth and Life Sciences Council (NWO-ALW VIDI 864.03.002, R.C.A. and G.J.C.V.), the Research Foundation-Flanders, the Interuniversity Attraction Poles (IAP7/07), the Belgian Association against Cancer and the Concerted Research Actions (GOA) of Ghent University (S.J. and K.V.)

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KW - distinct roles

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KW - genes

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