Tissue localization of retinoic acid receptor (RAR) active drugs

Azita Kouchmeshky, Timothy Goodman, Timothy Goodman, Peter McCaffery*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The retinoic acid (RA) signaling pathway is crucial for the control of embryonic development and also regulates function of several organ systems in the adult, including the central nervous system. The retinoic acid receptors (RARs) that mediate the majority of the functions of RA can promote proliferation, differentiation, morphogenesis and cell survival. Dysregulation of this signaling pathway has been considered in the pathophysiology of various diseases including neurodegenerative disorders such Alzheimer's disease and amyotrophic lateral sclerosis. Thus, drugs targeted to the RARs have been proposed as treatments for such diseases. Understanding how these drugs distribute in the body is essential to determine their potential effectiveness. However measuring tissue levels of what are often lipophilic drugs can be difficult. Here we describe an indirect measurement of RAR ligand tissue distribution after intraperitoneal injection into rodents that uses a sensitive RA reporter cell line.

Original languageEnglish
Title of host publicationMethods in Enzymology
Subtitle of host publicationRetinoid Signaling Pathways, Volume
EditorsEhmke Pohl
Place of PublicationLondon
PublisherElsevier
Chapter19
Pages513-538
Number of pages26
Volume637
Edition1
ISBN (Electronic) 9780128201473
ISBN (Print)9780128201442
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Enzymology
Volume637
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Animal
  • Drug
  • Extraction
  • Neurodegenerative disease
  • Quantitation
  • Reporter
  • Retinoic acid receptor
  • Sil-15
  • Tissue

Fingerprint

Dive into the research topics of 'Tissue localization of retinoic acid receptor (RAR) active drugs'. Together they form a unique fingerprint.

Cite this