TRANSCRIPTION MAP OF THE EARLY REGION OF THE STREPTOMYCES BACTERIOPHAGE PHI-C31

C J INGHAM, Margaret Caroline MacHin Smith

Research output: Contribution to journalArticlepeer-review

Abstract

Streptomyces coelicolor A3(2), lysogenised by the temperature-sensitive c(ts1) mutant of phiC31, can be synchronously induced into the lytic cycle by heat treatment. A transcription map of 10 kb of the phiC31 early gene cluster was deduced using low-resolution S1 nuclease mapping of RNA prepared 10 min after induction. At least nine early transcripts, early (e)RNAs 1-9, were localised reading exclusively rightwards with respect to the standard physical map of phiC31. The mRNAs were extensively overlapping, frequently initiating at the same place but terminating at different sites, and vice versa. Gene expression during the lytic cycle was tightly regulated; no transcription was observed before induction. Transcription was maximal at 10 min post-induction, and at 20 min, eRNAs 5 and 6 persisted whilst eRNAs 7-9 were severely reduced or absent. The pattern of transcription of the early region is consistent with the simultaneous activation of a large number of promoters and differential termination efficiency.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalGene
Volume122
Issue number1
Publication statusPublished - 1 Dec 1992

Keywords

  • ACTINOPHAGE
  • GENE EXPRESSION
  • GENE REGULATION
  • PROMOTER
  • PHAGE
  • TERMINATION
  • PHAGE PHI-C31
  • CLONING
  • GENE
  • VECTORS

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