Transcriptome analysis of the synganglion from the brown dog tick, Rhipicephalus sanguineus

Kristin Lees, Debra Woods, Alan S Bowman (Corresponding Author)

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Tick control strategies rely heavily on chemicals (acaricides), most of which target the central nervous system. With increasing resistance, new acaricides are urgently needed but knowledge of tick neurobiology is surprisingly limited, notably the number of neural-specific gene sequences. One thousand and eight expressed sequence tags (ESTs) were obtained from a normalized cDNA library from Rhipicephalus sanguineus synganglia. Putative functional identities were assigned to 44% whereas 34% were unknown/novel sequences. Of particular interest were ESTs encoding a chitinase-like enzyme, an acetylcholinesterase and four transmembrane receptors including two glutamate-gated chloride channel receptors, a leucokinin-like receptor and a nicotinic acetylcholine receptor a-subunit. This study highlights the benefits of using both neural tissues and normalized libraries in an EST-approach for identifying potential acaricide targets expressed as rare transcripts.
Original languageEnglish
Pages (from-to)273-282
Number of pages10
JournalInsect Molecular Biology
Volume19
Issue number3
Early online date7 Dec 2009
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Rhipicephalus sanguineus
Acaricides
acaricides
Expressed Sequence Tags
Gene Expression Profiling
expressed sequence tags
transcriptomics
receptors
Tick Control
chloride channels
tick control
Chitinases
neurophysiology
cholinergic receptors
Neurobiology
Nicotinic Receptors
Ticks
Acetylcholinesterase
acetylcholinesterase
chitinase

Keywords

  • tick
  • EST
  • glutamate-gated chloride channel
  • chitinase
  • leucokinin
  • acaricide

Cite this

Transcriptome analysis of the synganglion from the brown dog tick, Rhipicephalus sanguineus. / Lees, Kristin; Woods, Debra; Bowman, Alan S (Corresponding Author).

In: Insect Molecular Biology, Vol. 19, No. 3, 06.2010, p. 273-282.

Research output: Contribution to journalArticle

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