TRANSFER OF HYBRID PLASMIDS BASED ON THE REPLICON PRR17 FROM ESCHERICHIA-COLI TO BACTEROIDES AND PREVOTELLA STRAINS

M BECHET, P PHEULPIN, H J FLINT, J MARTIN, H C DUBOURGUIER

Research output: Contribution to journalArticle

Abstract

New shuttle vectors based on a Prevotella ruminicola 9.5 kb cryptic plasmid (pRRI7) inserted within the Escherichia coli vector pKC71, carrying the Cc(r)/Em(r) Bacteroides marker, were constructed. These constructs (pKBR23-1 and pKBR23-2) were transferred into Bacteroides distasonis, Bacteroides thetaiotaomicron, Bacteroides uniforms and into P. ruminicola NCFB 2202 either by conjugal mobilization or by electroporation. Another pRRI7 derivative based on pKC72, PKBR23-3, was smaller (13.1 kb) and non-mobilizable. By electroporation, it was transferred to Bact. distasonis and P. ruminicola. Being derived from pRRI7 which is compatible with the shuttle plasmid pRRI207, the host/vector combination involving P. ruminicola NCFB 2202 and pKBR23-3 offers new possibilities for genetic investigations in rumen anaerobic bacteria after further introduction of a second readily selectable marker within pRRI207 or pKBR23-3.

Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalJournal of Applied Bacteriology
Volume74
Issue number5
Publication statusPublished - May 1993

Keywords

  • AGROBACTERIUM-TUMEFACIENS
  • RUMINAL STRAINS
  • RESISTANCE GENE
  • XYLANASE GENE
  • TI-PLASMIDS
  • RUMINICOLA
  • CLONING
  • EXPRESSION
  • UNIFORMIS
  • FRAGILIS

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