Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry

Garry Jonathan Rucklidge, G MILNE, B A MCGAW, Eric Milne, Simon Robins

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The rates of collagen turnover in different tissues have been estimated in growing rats previously exposed to gaseous O-18(2). The abundance of the stable isotope was measured using isotope ratio mass spectrometry following combustion of isolated collagen-derived hydroxyproline. Using this method, problems of label reutilization associated with radiolabelling methods are avoided. In general the results confirm the slow turnover rates with half-lives of total collagen in skin, muscle and gut of 74, 45 and 244 d, respectively. The use of cyanogen bromide digests of whole tissues followed by isolation of collagen type-specific peptides has allowed the comparison of turnover rates of collagen types I and III, indicating that collagen type III is turned over more rapidly than type I.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalBiochimica et Biophysica Acta
Volume1156
Issue number1
Publication statusPublished - 8 Dec 1992

Keywords

  • collagen turnover
  • isotope ratio mass spectrometry
  • turnover rate
  • collagen type
  • I-collagen
  • skin collagen
  • III-collagen
  • degradation
  • tissues
  • metabolism
  • invivo

Cite this

Rucklidge, G. J., MILNE, G., MCGAW, B. A., Milne, E., & Robins, S. (1992). Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry. Biochimica et Biophysica Acta, 1156(1), 57-61.

Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry. / Rucklidge, Garry Jonathan; MILNE, G ; MCGAW, B A ; Milne, Eric; Robins, Simon.

In: Biochimica et Biophysica Acta, Vol. 1156, No. 1, 08.12.1992, p. 57-61.

Research output: Contribution to journalArticle

Rucklidge, GJ, MILNE, G, MCGAW, BA, Milne, E & Robins, S 1992, 'Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry' Biochimica et Biophysica Acta, vol. 1156, no. 1, pp. 57-61.
Rucklidge GJ, MILNE G, MCGAW BA, Milne E, Robins S. Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry. Biochimica et Biophysica Acta. 1992 Dec 8;1156(1):57-61.
Rucklidge, Garry Jonathan ; MILNE, G ; MCGAW, B A ; Milne, Eric ; Robins, Simon. / Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry. In: Biochimica et Biophysica Acta. 1992 ; Vol. 1156, No. 1. pp. 57-61.
@article{147f2c0b3ef34e95a868bb73ce1f6228,
title = "Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry",
abstract = "The rates of collagen turnover in different tissues have been estimated in growing rats previously exposed to gaseous O-18(2). The abundance of the stable isotope was measured using isotope ratio mass spectrometry following combustion of isolated collagen-derived hydroxyproline. Using this method, problems of label reutilization associated with radiolabelling methods are avoided. In general the results confirm the slow turnover rates with half-lives of total collagen in skin, muscle and gut of 74, 45 and 244 d, respectively. The use of cyanogen bromide digests of whole tissues followed by isolation of collagen type-specific peptides has allowed the comparison of turnover rates of collagen types I and III, indicating that collagen type III is turned over more rapidly than type I.",
keywords = "collagen turnover, isotope ratio mass spectrometry, turnover rate, collagen type, I-collagen, skin collagen, III-collagen, degradation, tissues, metabolism, invivo",
author = "Rucklidge, {Garry Jonathan} and G MILNE and MCGAW, {B A} and Eric Milne and Simon Robins",
year = "1992",
month = "12",
day = "8",
language = "English",
volume = "1156",
pages = "57--61",
journal = "Biochimica et Biophysica Acta",
issn = "0006-3002",
number = "1",

}

TY - JOUR

T1 - Turnover Rates of Different Collagen Types Measured by Isotope Ratio Mass-Spectrometry

AU - Rucklidge, Garry Jonathan

AU - MILNE, G

AU - MCGAW, B A

AU - Milne, Eric

AU - Robins, Simon

PY - 1992/12/8

Y1 - 1992/12/8

N2 - The rates of collagen turnover in different tissues have been estimated in growing rats previously exposed to gaseous O-18(2). The abundance of the stable isotope was measured using isotope ratio mass spectrometry following combustion of isolated collagen-derived hydroxyproline. Using this method, problems of label reutilization associated with radiolabelling methods are avoided. In general the results confirm the slow turnover rates with half-lives of total collagen in skin, muscle and gut of 74, 45 and 244 d, respectively. The use of cyanogen bromide digests of whole tissues followed by isolation of collagen type-specific peptides has allowed the comparison of turnover rates of collagen types I and III, indicating that collagen type III is turned over more rapidly than type I.

AB - The rates of collagen turnover in different tissues have been estimated in growing rats previously exposed to gaseous O-18(2). The abundance of the stable isotope was measured using isotope ratio mass spectrometry following combustion of isolated collagen-derived hydroxyproline. Using this method, problems of label reutilization associated with radiolabelling methods are avoided. In general the results confirm the slow turnover rates with half-lives of total collagen in skin, muscle and gut of 74, 45 and 244 d, respectively. The use of cyanogen bromide digests of whole tissues followed by isolation of collagen type-specific peptides has allowed the comparison of turnover rates of collagen types I and III, indicating that collagen type III is turned over more rapidly than type I.

KW - collagen turnover

KW - isotope ratio mass spectrometry

KW - turnover rate

KW - collagen type

KW - I-collagen

KW - skin collagen

KW - III-collagen

KW - degradation

KW - tissues

KW - metabolism

KW - invivo

M3 - Article

VL - 1156

SP - 57

EP - 61

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

SN - 0006-3002

IS - 1

ER -