Unique haplotypes of co-segregating major histocompatibility class II A and class II B alleles in Atlantic salmon (Salmo salar) give rise to diverse class II genotypes

Rene Josephus Maria Stet, B. de Vries, K. Mudde, T. T. Hermsen, J. van Heerwaarden, B. P. Shum, U. Grimholt

    Research output: Contribution to journalArticle

    111 Citations (Scopus)

    Abstract

    Sequence-based typing of a breeding population (GI) consisting of 84 Atlantic salmon individuals revealed the presence of 7 Sasa-DAA and 7 Sasa-DAB expressed alleles. Subsequent typing of 1,182 individuals belonging to 33 families showed that Sasa-DAA and Sasa-DAB segregate as haplotypes. In total seven unique haplotypes were established, with frequencies in the population studied ranging from 0.01 to 0.49. Each haplotype is characterized by a unique minisatellite marker size embedded in the 3' untranslated region of the Sasa-DAA gene. These data corroborate the fact that Atlantic salmon express a single class II locus, consisting of tightly linked class II A and class B genes. The seven haplotypes give rise to 15 genotypes with frequencies varying between 0.01 and 0.23; 21 class II homozygous individuals were present in the GI population. We also studied the frequency distribution in another breeding population (G4, n=374) using the minisatellite marker. Only one new marker size was present, suggesting the presence of one new class H haplotype. The marker frequency distribution in the G4 population differed markedly from the GI population. The genomic organizations of two Sasa-DAA and Sasa-DAB alleles were deter-mined, and supported the notion that these alleles belong to the same locus. In contrast to other studies of salmonid class II sequences, phylogenetic analyses of brown trout and Atlantic class II A and class II B sequences provided support for trans-species polymorphism.

    Original languageEnglish
    Pages (from-to)320-331
    Number of pages11
    JournalImmunogenetics
    Volume54
    Issue number5
    DOIs
    Publication statusPublished - Aug 2002

    Keywords

    • Atlantic salmon
    • MHC
    • class II
    • segregation
    • genotyping
    • CHAIN-ENCODING GENES
    • COMPLEX CLASS-I
    • MHC CLASS-I
    • A-GENES
    • LINKAGE RELATIONSHIPS
    • PACIFIC SALMON
    • BETA-CHAIN
    • SEQUENCE-ANALYSIS
    • CRYSTAL-STRUCTURE
    • CICHLID FISHES

    Cite this

    Unique haplotypes of co-segregating major histocompatibility class II A and class II B alleles in Atlantic salmon (Salmo salar) give rise to diverse class II genotypes. / Stet, Rene Josephus Maria; de Vries, B.; Mudde, K.; Hermsen, T. T.; van Heerwaarden, J.; Shum, B. P.; Grimholt, U.

    In: Immunogenetics, Vol. 54, No. 5, 08.2002, p. 320-331.

    Research output: Contribution to journalArticle

    Stet, Rene Josephus Maria ; de Vries, B. ; Mudde, K. ; Hermsen, T. T. ; van Heerwaarden, J. ; Shum, B. P. ; Grimholt, U. / Unique haplotypes of co-segregating major histocompatibility class II A and class II B alleles in Atlantic salmon (Salmo salar) give rise to diverse class II genotypes. In: Immunogenetics. 2002 ; Vol. 54, No. 5. pp. 320-331.
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    title = "Unique haplotypes of co-segregating major histocompatibility class II A and class II B alleles in Atlantic salmon (Salmo salar) give rise to diverse class II genotypes",
    abstract = "Sequence-based typing of a breeding population (GI) consisting of 84 Atlantic salmon individuals revealed the presence of 7 Sasa-DAA and 7 Sasa-DAB expressed alleles. Subsequent typing of 1,182 individuals belonging to 33 families showed that Sasa-DAA and Sasa-DAB segregate as haplotypes. In total seven unique haplotypes were established, with frequencies in the population studied ranging from 0.01 to 0.49. Each haplotype is characterized by a unique minisatellite marker size embedded in the 3' untranslated region of the Sasa-DAA gene. These data corroborate the fact that Atlantic salmon express a single class II locus, consisting of tightly linked class II A and class B genes. The seven haplotypes give rise to 15 genotypes with frequencies varying between 0.01 and 0.23; 21 class II homozygous individuals were present in the GI population. We also studied the frequency distribution in another breeding population (G4, n=374) using the minisatellite marker. Only one new marker size was present, suggesting the presence of one new class H haplotype. The marker frequency distribution in the G4 population differed markedly from the GI population. The genomic organizations of two Sasa-DAA and Sasa-DAB alleles were deter-mined, and supported the notion that these alleles belong to the same locus. In contrast to other studies of salmonid class II sequences, phylogenetic analyses of brown trout and Atlantic class II A and class II B sequences provided support for trans-species polymorphism.",
    keywords = "Atlantic salmon, MHC, class II, segregation, genotyping, CHAIN-ENCODING GENES, COMPLEX CLASS-I, MHC CLASS-I, A-GENES, LINKAGE RELATIONSHIPS, PACIFIC SALMON, BETA-CHAIN, SEQUENCE-ANALYSIS, CRYSTAL-STRUCTURE, CICHLID FISHES",
    author = "Stet, {Rene Josephus Maria} and {de Vries}, B. and K. Mudde and Hermsen, {T. T.} and {van Heerwaarden}, J. and Shum, {B. P.} and U. Grimholt",
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    T1 - Unique haplotypes of co-segregating major histocompatibility class II A and class II B alleles in Atlantic salmon (Salmo salar) give rise to diverse class II genotypes

    AU - Stet, Rene Josephus Maria

    AU - de Vries, B.

    AU - Mudde, K.

    AU - Hermsen, T. T.

    AU - van Heerwaarden, J.

    AU - Shum, B. P.

    AU - Grimholt, U.

    PY - 2002/8

    Y1 - 2002/8

    N2 - Sequence-based typing of a breeding population (GI) consisting of 84 Atlantic salmon individuals revealed the presence of 7 Sasa-DAA and 7 Sasa-DAB expressed alleles. Subsequent typing of 1,182 individuals belonging to 33 families showed that Sasa-DAA and Sasa-DAB segregate as haplotypes. In total seven unique haplotypes were established, with frequencies in the population studied ranging from 0.01 to 0.49. Each haplotype is characterized by a unique minisatellite marker size embedded in the 3' untranslated region of the Sasa-DAA gene. These data corroborate the fact that Atlantic salmon express a single class II locus, consisting of tightly linked class II A and class B genes. The seven haplotypes give rise to 15 genotypes with frequencies varying between 0.01 and 0.23; 21 class II homozygous individuals were present in the GI population. We also studied the frequency distribution in another breeding population (G4, n=374) using the minisatellite marker. Only one new marker size was present, suggesting the presence of one new class H haplotype. The marker frequency distribution in the G4 population differed markedly from the GI population. The genomic organizations of two Sasa-DAA and Sasa-DAB alleles were deter-mined, and supported the notion that these alleles belong to the same locus. In contrast to other studies of salmonid class II sequences, phylogenetic analyses of brown trout and Atlantic class II A and class II B sequences provided support for trans-species polymorphism.

    AB - Sequence-based typing of a breeding population (GI) consisting of 84 Atlantic salmon individuals revealed the presence of 7 Sasa-DAA and 7 Sasa-DAB expressed alleles. Subsequent typing of 1,182 individuals belonging to 33 families showed that Sasa-DAA and Sasa-DAB segregate as haplotypes. In total seven unique haplotypes were established, with frequencies in the population studied ranging from 0.01 to 0.49. Each haplotype is characterized by a unique minisatellite marker size embedded in the 3' untranslated region of the Sasa-DAA gene. These data corroborate the fact that Atlantic salmon express a single class II locus, consisting of tightly linked class II A and class B genes. The seven haplotypes give rise to 15 genotypes with frequencies varying between 0.01 and 0.23; 21 class II homozygous individuals were present in the GI population. We also studied the frequency distribution in another breeding population (G4, n=374) using the minisatellite marker. Only one new marker size was present, suggesting the presence of one new class H haplotype. The marker frequency distribution in the G4 population differed markedly from the GI population. The genomic organizations of two Sasa-DAA and Sasa-DAB alleles were deter-mined, and supported the notion that these alleles belong to the same locus. In contrast to other studies of salmonid class II sequences, phylogenetic analyses of brown trout and Atlantic class II A and class II B sequences provided support for trans-species polymorphism.

    KW - Atlantic salmon

    KW - MHC

    KW - class II

    KW - segregation

    KW - genotyping

    KW - CHAIN-ENCODING GENES

    KW - COMPLEX CLASS-I

    KW - MHC CLASS-I

    KW - A-GENES

    KW - LINKAGE RELATIONSHIPS

    KW - PACIFIC SALMON

    KW - BETA-CHAIN

    KW - SEQUENCE-ANALYSIS

    KW - CRYSTAL-STRUCTURE

    KW - CICHLID FISHES

    U2 - 10.1007/s00251-002-0477-1

    DO - 10.1007/s00251-002-0477-1

    M3 - Article

    VL - 54

    SP - 320

    EP - 331

    JO - Immunogenetics

    JF - Immunogenetics

    SN - 0093-7711

    IS - 5

    ER -