Use of differential display to detect changes in gene expression in the intermediate snail host Biomphalaria glabrata upon infection with Schistosoma mansoni

A E Lockyer, C S Jones, L R Noble, D Rollinson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Changes in gene expression in Biomphalaria glabrata following infection with Schistosoma mansoni have been investigated using a modified differential display approach. RNA was extracted from ovotestis, mantle tissue and anterior nephridium of control and exposed snails at 2 time-points (4 h and 24 h) post-exposure and analysed by RNA fingerprinting. A number of transcripts were identified; some novel and some homologous to mRNAs in GenEMBL that were previously unknown in B. glabrata. Down regulation of one 241 bp mRNA expressed sequence fragment - with an open reading frame showing 48% identity to a cytochrome p450 over 80 residues - has been confirmed using semi-quantitative RT-PCR. Preliminary classification of B. glabrata cyp450 sequence shows it to fall into CLAN 2 of the cytochrome p450 superfamily. Differential display has been successful in identifying changes in gene expression in Biomphalaria glabrata upon infection with Schistosoma mansoni and promises to be a useful technique for the investigation of the interaction between host and parasite.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalParasitology
Volume120
Publication statusPublished - 2000

Keywords

  • Biomphalaria glabrata
  • Schistosoma mansoni
  • differential display
  • cytochrome p450
  • polymerase chain reaction
  • GLYCINE CLEAVAGE SYSTEM
  • ARBITRARILY PRIMED PCR
  • H-PROTEIN
  • PLASMA KALLIKREIN
  • FASCIOLA-HEPATICA
  • MESSENGER-RNA
  • CYTOCHROME-P450
  • CDNA
  • CLONING
  • SEQUENCES

Cite this

Use of differential display to detect changes in gene expression in the intermediate snail host Biomphalaria glabrata upon infection with Schistosoma mansoni. / Lockyer, A E ; Jones, C S ; Noble, L R ; Rollinson, D .

In: Parasitology, Vol. 120, 2000, p. 399-407.

Research output: Contribution to journalArticle

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AB - Changes in gene expression in Biomphalaria glabrata following infection with Schistosoma mansoni have been investigated using a modified differential display approach. RNA was extracted from ovotestis, mantle tissue and anterior nephridium of control and exposed snails at 2 time-points (4 h and 24 h) post-exposure and analysed by RNA fingerprinting. A number of transcripts were identified; some novel and some homologous to mRNAs in GenEMBL that were previously unknown in B. glabrata. Down regulation of one 241 bp mRNA expressed sequence fragment - with an open reading frame showing 48% identity to a cytochrome p450 over 80 residues - has been confirmed using semi-quantitative RT-PCR. Preliminary classification of B. glabrata cyp450 sequence shows it to fall into CLAN 2 of the cytochrome p450 superfamily. Differential display has been successful in identifying changes in gene expression in Biomphalaria glabrata upon infection with Schistosoma mansoni and promises to be a useful technique for the investigation of the interaction between host and parasite.

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KW - cytochrome p450

KW - polymerase chain reaction

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KW - FASCIOLA-HEPATICA

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KW - CDNA

KW - CLONING

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