Abstract
Changes in gene expression in Biomphalaria glabrata following infection with Schistosoma mansoni have been investigated using a modified differential display approach. RNA was extracted from ovotestis, mantle tissue and anterior nephridium of control and exposed snails at 2 time-points (4 h and 24 h) post-exposure and analysed by RNA fingerprinting. A number of transcripts were identified; some novel and some homologous to mRNAs in GenEMBL that were previously unknown in B. glabrata. Down regulation of one 241 bp mRNA expressed sequence fragment - with an open reading frame showing 48% identity to a cytochrome p450 over 80 residues - has been confirmed using semi-quantitative RT-PCR. Preliminary classification of B. glabrata cyp450 sequence shows it to fall into CLAN 2 of the cytochrome p450 superfamily. Differential display has been successful in identifying changes in gene expression in Biomphalaria glabrata upon infection with Schistosoma mansoni and promises to be a useful technique for the investigation of the interaction between host and parasite.
Original language | English |
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Pages (from-to) | 399-407 |
Number of pages | 9 |
Journal | Parasitology |
Volume | 120 |
Publication status | Published - 2000 |
Keywords
- Biomphalaria glabrata
- Schistosoma mansoni
- differential display
- cytochrome p450
- polymerase chain reaction
- GLYCINE CLEAVAGE SYSTEM
- ARBITRARILY PRIMED PCR
- H-PROTEIN
- PLASMA KALLIKREIN
- FASCIOLA-HEPATICA
- MESSENGER-RNA
- CYTOCHROME-P450
- CDNA
- CLONING
- SEQUENCES