Use of restriction fragment length polymorphism to distinguish between salmon species

Valerie Joan Stevens; Georgina Louise Hold; Elizabeth Pryde; H Rehbein; J Quinteiro; M Rey-Mendez; C G Sotelo; Ricardo I.  Perez-Martin; Gildomar Alves Santos; C Rosa

doi:10.1021/jf991213e

Use of restriction fragment length polymorphism to distinguish between salmon species

Valerie Joan Stevens, Georgina Louise Hold, Elizabeth Pryde, H Rehbein, J Quinteiro, M Rey-Mendez, C G Sotelo, Ricardo I. Perez-Martin, Gildomar Alves Santos, C Rosa

Research output: Contribution to journal › Article › peer-review

121 Citations (Scopus)

Abstract

Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.

Original language	English
Pages (from-to)	2184-2188
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	48
Issue number	6
DOIs	https://doi.org/10.1021/jf991213e
Publication status	Published - 1 Jun 2000

Keywords

animals
base sequence
molecular sequence data
Oncorhynchus
Oncorhynchus keta
Oncorhynchus kisutch
Oncorhynchus mykiss
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
restriction mapping
salmon
Salmonidae
sequence alignment
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
trout
seafood products
canned tuna
authentication
PCR-RFLP
species identification

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10.1021/jf991213e

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@article{a3c05ea944da4567877b0978b5235a70,

title = "Use of restriction fragment length polymorphism to distinguish between salmon species",

abstract = "Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.",

keywords = "animals, base sequence, molecular sequence data, Oncorhynchus, Oncorhynchus keta, Oncorhynchus kisutch, Oncorhynchus mykiss, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, restriction mapping, salmon, Salmonidae, sequence alignment, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, trout, seafood products, canned tuna, authentication, PCR-RFLP, species identification",

author = "Stevens, {Valerie Joan} and Hold, {Georgina Louise} and Elizabeth Pryde and H Rehbein and J Quinteiro and M Rey-Mendez and Sotelo, {C G} and Perez-Martin, {Ricardo I.} and Santos, {Gildomar Alves} and C Rosa",

year = "2000",

month = jun,

day = "1",

doi = "10.1021/jf991213e",

language = "English",

volume = "48",

pages = "2184--2188",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Use of restriction fragment length polymorphism to distinguish between salmon species

AU - Stevens, Valerie Joan

AU - Hold, Georgina Louise

AU - Pryde, Elizabeth

AU - Rehbein, H

AU - Quinteiro, J

AU - Rey-Mendez, M

AU - Sotelo, C G

AU - Perez-Martin, Ricardo I.

AU - Santos, Gildomar Alves

AU - Rosa, C

PY - 2000/6/1

Y1 - 2000/6/1

N2 - Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.

AB - Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.

KW - animals

KW - base sequence

KW - molecular sequence data

KW - Oncorhynchus

KW - Oncorhynchus keta

KW - Oncorhynchus kisutch

KW - Oncorhynchus mykiss

KW - Polymerase Chain Reaction

KW - Polymorphism, Restriction Fragment Length

KW - restriction mapping

KW - salmon

KW - Salmonidae

KW - sequence alignment

KW - Sequence Analysis, DNA

KW - Sequence Homology, Nucleic Acid

KW - trout

KW - seafood products

KW - canned tuna

KW - authentication

KW - PCR-RFLP

KW - species identification

U2 - 10.1021/jf991213e

DO - 10.1021/jf991213e

M3 - Article

C2 - 10888519

SN - 0021-8561

VL - 48

SP - 2184

EP - 2188

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 6

ER -

Use of restriction fragment length polymorphism to distinguish between salmon species

Abstract

Keywords

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