Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii

Leena Al-Hassan, Andres Opazo, Bruno S Lopes, Hadir El Mahallawy, Sebastian G B Amyes

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined according to British Society of Antimicrobial Chemotherapy (BSAC) guidelines. PCR and sequencing of blaOXA-58 and the upstream and downstream regions was performed to determine the genetic environment. The two isolates were positive for the intrinsic blaOXA-64 gene, and the MICs for isolates AB-14298 and AB-P67 were 8mg/L and 64mg/L for imipenem and 2mg/L and 16mg/L for meropenem, respectively. The blaOXA-58 gene in AB-14298 was flanked by ISAba3 interrupted with IS1006, whereas AB-P67 had ISAba3 interrupted by IS1008, both belonging to the IS6 family of insertion sequences. In conclusion, both IS1006 and IS1008 provided suitable promoter sequences for expression of the downstream blaOXA-58 gene.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalJournal of global antimicrobial resistance
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 2015

Fingerprint

Acinetobacter baumannii
Carbapenems
meropenem
Imipenem
Insertional Mutagenesis
Microbial Sensitivity Tests
Genes
Egypt
Pulsed Field Gel Electrophoresis
Guidelines
Pediatrics
Drug Therapy
Polymerase Chain Reaction
Neoplasms

Keywords

  • Acinetobacter baumannii
  • blaOXA-58
  • Carbapenem resistance
  • Genetic environment
  • Insertion sequences
  • IS6 family

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii. / Al-Hassan, Leena; Opazo, Andres; Lopes, Bruno S; Mahallawy, Hadir El; Amyes, Sebastian G B.

In: Journal of global antimicrobial resistance, Vol. 3, No. 1, 03.2015, p. 5-8.

Research output: Contribution to journalArticle

Al-Hassan, Leena ; Opazo, Andres ; Lopes, Bruno S ; Mahallawy, Hadir El ; Amyes, Sebastian G B. / Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii. In: Journal of global antimicrobial resistance. 2015 ; Vol. 3, No. 1. pp. 5-8.
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abstract = "The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined according to British Society of Antimicrobial Chemotherapy (BSAC) guidelines. PCR and sequencing of blaOXA-58 and the upstream and downstream regions was performed to determine the genetic environment. The two isolates were positive for the intrinsic blaOXA-64 gene, and the MICs for isolates AB-14298 and AB-P67 were 8mg/L and 64mg/L for imipenem and 2mg/L and 16mg/L for meropenem, respectively. The blaOXA-58 gene in AB-14298 was flanked by ISAba3 interrupted with IS1006, whereas AB-P67 had ISAba3 interrupted by IS1008, both belonging to the IS6 family of insertion sequences. In conclusion, both IS1006 and IS1008 provided suitable promoter sequences for expression of the downstream blaOXA-58 gene.",
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AU - Amyes, Sebastian G B

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