Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii

Leena Al-Hassan; Andres Opazo; Bruno S Lopes; Hadir El Mahallawy; Sebastian G B Amyes

doi:10.1016/j.jgar.2014.10.003

Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii

Leena Al-Hassan, Andres Opazo, Bruno S Lopes, Hadir El Mahallawy, Sebastian G B Amyes

Applied Medicine

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined according to British Society of Antimicrobial Chemotherapy (BSAC) guidelines. PCR and sequencing of blaOXA-58 and the upstream and downstream regions was performed to determine the genetic environment. The two isolates were positive for the intrinsic blaOXA-64 gene, and the MICs for isolates AB-14298 and AB-P67 were 8mg/L and 64mg/L for imipenem and 2mg/L and 16mg/L for meropenem, respectively. The blaOXA-58 gene in AB-14298 was flanked by ISAba3 interrupted with IS1006, whereas AB-P67 had ISAba3 interrupted by IS1008, both belonging to the IS6 family of insertion sequences. In conclusion, both IS1006 and IS1008 provided suitable promoter sequences for expression of the downstream blaOXA-58 gene.

Original language	English
Pages (from-to)	5-8
Number of pages	4
Journal	Journal of global antimicrobial resistance
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.jgar.2014.10.003
Publication status	Published - Mar 2015

Bibliographical note

The authors are thankful to the hospital staff at The Children’s Cancer Hospital and The National Cancer Institute (Cairo, Egypt) for providing the samples and allowing part of the work to be
undertaken at their centres.

Keywords

Acinetobacter baumannii
blaOXA-58
Carbapenem resistance
Genetic environment
Insertion sequences
IS6 family

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii",

abstract = "The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined according to British Society of Antimicrobial Chemotherapy (BSAC) guidelines. PCR and sequencing of blaOXA-58 and the upstream and downstream regions was performed to determine the genetic environment. The two isolates were positive for the intrinsic blaOXA-64 gene, and the MICs for isolates AB-14298 and AB-P67 were 8mg/L and 64mg/L for imipenem and 2mg/L and 16mg/L for meropenem, respectively. The blaOXA-58 gene in AB-14298 was flanked by ISAba3 interrupted with IS1006, whereas AB-P67 had ISAba3 interrupted by IS1008, both belonging to the IS6 family of insertion sequences. In conclusion, both IS1006 and IS1008 provided suitable promoter sequences for expression of the downstream blaOXA-58 gene.",

keywords = "Acinetobacter baumannii, blaOXA-58, Carbapenem resistance, Genetic environment, Insertion sequences, IS6 family",

author = "Leena Al-Hassan and Andres Opazo and Lopes, {Bruno S} and Mahallawy, {Hadir El} and Amyes, {Sebastian G B}",

note = "The authors are thankful to the hospital staff at The Children{\textquoteright}s Cancer Hospital and The National Cancer Institute (Cairo, Egypt) for providing the samples and allowing part of the work to be undertaken at their centres.",

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AU - Al-Hassan, Leena

AU - Opazo, Andres

AU - Lopes, Bruno S

AU - Mahallawy, Hadir El

AU - Amyes, Sebastian G B

N1 - The authors are thankful to the hospital staff at The Children’s Cancer Hospital and The National Cancer Institute (Cairo, Egypt) for providing the samples and allowing part of the work to be undertaken at their centres.

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N2 - The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined according to British Society of Antimicrobial Chemotherapy (BSAC) guidelines. PCR and sequencing of blaOXA-58 and the upstream and downstream regions was performed to determine the genetic environment. The two isolates were positive for the intrinsic blaOXA-64 gene, and the MICs for isolates AB-14298 and AB-P67 were 8mg/L and 64mg/L for imipenem and 2mg/L and 16mg/L for meropenem, respectively. The blaOXA-58 gene in AB-14298 was flanked by ISAba3 interrupted with IS1006, whereas AB-P67 had ISAba3 interrupted by IS1008, both belonging to the IS6 family of insertion sequences. In conclusion, both IS1006 and IS1008 provided suitable promoter sequences for expression of the downstream blaOXA-58 gene.

AB - The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined according to British Society of Antimicrobial Chemotherapy (BSAC) guidelines. PCR and sequencing of blaOXA-58 and the upstream and downstream regions was performed to determine the genetic environment. The two isolates were positive for the intrinsic blaOXA-64 gene, and the MICs for isolates AB-14298 and AB-P67 were 8mg/L and 64mg/L for imipenem and 2mg/L and 16mg/L for meropenem, respectively. The blaOXA-58 gene in AB-14298 was flanked by ISAba3 interrupted with IS1006, whereas AB-P67 had ISAba3 interrupted by IS1008, both belonging to the IS6 family of insertion sequences. In conclusion, both IS1006 and IS1008 provided suitable promoter sequences for expression of the downstream blaOXA-58 gene.

KW - Acinetobacter baumannii

KW - blaOXA-58

KW - Carbapenem resistance

KW - Genetic environment

KW - Insertion sequences

KW - IS6 family

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EP - 8

JO - Journal of global antimicrobial resistance

JF - Journal of global antimicrobial resistance

IS - 1

ER -

Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii

Abstract

Bibliographical note

Keywords

UN SDGs

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