VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm

Lynda Erskine, Susan Reijntjes, Thomas Pratt, Laura Denti, Quenten Schwarz, Joaquim Vieira, Bennett Alakakone, Derryck Shewan, Christiana Ruhrberg

Research output: Contribution to journalArticle

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Abstract

During development, the axons of retinal ganglion cell (RGC) neurons must decide whether to cross or avoid the midline at the optic chiasm to project to targets on both sides of the brain. By combining genetic analyses with in vitro assays, we show that neuropilin 1 (NRP1) promotes contralateral RGC projection in mammals. Unexpectedly, the NRP1 ligand involved is not an axon guidance cue of the class 3 semaphorin family, but VEGF164, the neuropilin-binding isoform of the classical vascular growth factor VEGF-A. VEGF164 is expressed at the chiasm midline and is required for normal contralateral growth in vivo. In outgrowth and growth cone turning assays, VEGF164 acts directly on NRP1-expressing contralateral RGCs to provide growth-promoting and chemoattractive signals. These findings have identified a permissive midline signal for axons at the chiasm midline and provide in vivo evidence that VEGF-A is an essential axon guidance cue.
Original languageEnglish
Pages (from-to)951-965
Number of pages15
JournalNeuron
Volume70
Issue number5
Early online date8 Jun 2011
DOIs
Publication statusPublished - 9 Jun 2011

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Neuropilin-1
Optic Chiasm
Vascular Endothelial Growth Factor A
Axons
Retinal Ganglion Cells
Cues
Neuropilins
Semaphorins
Growth Cones
Growth
Mammals
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Ligands
Neurons
Brain
Axon Guidance

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Erskine, L., Reijntjes, S., Pratt, T., Denti, L., Schwarz, Q., Vieira, J., ... Ruhrberg, C. (2011). VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. Neuron, 70(5), 951-965. https://doi.org/10.1016/j.neuron.2011.02.052

VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. / Erskine, Lynda; Reijntjes, Susan; Pratt, Thomas; Denti, Laura; Schwarz, Quenten; Vieira, Joaquim; Alakakone, Bennett; Shewan, Derryck; Ruhrberg, Christiana.

In: Neuron, Vol. 70, No. 5, 09.06.2011, p. 951-965.

Research output: Contribution to journalArticle

Erskine, L, Reijntjes, S, Pratt, T, Denti, L, Schwarz, Q, Vieira, J, Alakakone, B, Shewan, D & Ruhrberg, C 2011, 'VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm', Neuron, vol. 70, no. 5, pp. 951-965. https://doi.org/10.1016/j.neuron.2011.02.052
Erskine, Lynda ; Reijntjes, Susan ; Pratt, Thomas ; Denti, Laura ; Schwarz, Quenten ; Vieira, Joaquim ; Alakakone, Bennett ; Shewan, Derryck ; Ruhrberg, Christiana. / VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. In: Neuron. 2011 ; Vol. 70, No. 5. pp. 951-965.
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