Vitamin D receptor gene variants and esophageal adenocarcinoma risk: A population-based case-control study

C. K. Chang; H. G. Mulholland; M. M. Cantwell; L. A. Anderson; B. T. Johnston; A. J. McKnight; P. D. Thompson; R. G.P. Watson; L. J. Murray

doi:10.1007/s12029-011-9322-9

Vitamin D receptor gene variants and esophageal adenocarcinoma risk: A population-based case-control study

C. K. Chang, H. G. Mulholland, M. M. Cantwell, L. A. Anderson, B. T. Johnston, A. J. McKnight, P. D. Thompson, R. G.P. Watson, L. J. Murray^* (Corresponding Author)

^*Corresponding author for this work

Applied Health Sciences

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Purpose: Polymorphisms in the vitamin D receptor (VDR) gene may be of etiological importance in determining cancer risk. The aim of this study was to assess the association between common VDR gene polymorphisms and esophageal adenocarcinoma (EAC) risk in an all-Ireland population-based case-control study. Methods: EAC cases and frequency-matched controls by age and gender recruited between March 2002 and December 2004 throughout Ireland were included. Participants were interviewed, and a blood sample collected for DNA extraction. Twenty-seven single nucleotide polymorphisms in the VDR gene were genotyped using Sequenom or TaqMan assays while the poly(A) microsatellite was genotyped by fluorescent fragment analysis. Unconditional logistic regression was applied to assess the association between VDR polymorphisms and EAC risk. Results: A total of 224 cases of EAC and 256 controls were involved in analyses. After adjustment for potential confounders, TT homozygotes at rs2238139 and rs2107301 had significantly reduced risks of EAC compared with CC homozygotes. In contrast, SS alleles of the poly(A) microsatellite had significantly elevated risks of EAC compared with SL/LL alleles. However, following permutation analyses to adjust for multiple comparisons, no significant associations were observed between any VDR gene polymorphism and EAC risk. Conclusions: VDR gene polymorphisms were not significantly associated with EAC development in this Irish population. Confirmation is required from larger studies.

Original language	English
Pages (from-to)	512-517
Number of pages	6
Journal	Journal of Gastrointestinal Cancer
Volume	43
Early online date	27 Sept 2011
DOIs	https://doi.org/10.1007/s12029-011-9322-9
Publication status	Published - 2012

Bibliographical note

Acknowledgments
The FINBAR study group members include LJ Murray (Queen’s University Belfast), LA Anderson (Queen’s University Belfast), BT Johnston (Belfast Health & Social Care Trust), RGP Watson (Belfast Health & Social Care Trust), J McGuigan (Belfast Health & Social Care Trust), HR Ferguson (Belfast Health & Social Care Trust), SJ Murphy (St Vincent’s Hospital Dublin), JV Reynolds (St James’ Hospital, Dublin) and H Comber (National Cancer Registry of Ireland). We appreciate the contributions made by the study participants, their families and all who assisted with the study, particularly the Northern Ireland Cancer Registry and National Cancer Registry Cork. This research was supported by funding from the Ulster Cancer Foundation, the Northern Ireland R&D office and the Health Research Board.

Keywords

Case-control studies
Esophageal adenocarcinoma
Polymorphism
Single nucleotide
Vitamin D receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

@article{63ab8333352a4da087f0153b82f576c5,

title = "Vitamin D receptor gene variants and esophageal adenocarcinoma risk: A population-based case-control study",

abstract = "Purpose: Polymorphisms in the vitamin D receptor (VDR) gene may be of etiological importance in determining cancer risk. The aim of this study was to assess the association between common VDR gene polymorphisms and esophageal adenocarcinoma (EAC) risk in an all-Ireland population-based case-control study. Methods: EAC cases and frequency-matched controls by age and gender recruited between March 2002 and December 2004 throughout Ireland were included. Participants were interviewed, and a blood sample collected for DNA extraction. Twenty-seven single nucleotide polymorphisms in the VDR gene were genotyped using Sequenom or TaqMan assays while the poly(A) microsatellite was genotyped by fluorescent fragment analysis. Unconditional logistic regression was applied to assess the association between VDR polymorphisms and EAC risk. Results: A total of 224 cases of EAC and 256 controls were involved in analyses. After adjustment for potential confounders, TT homozygotes at rs2238139 and rs2107301 had significantly reduced risks of EAC compared with CC homozygotes. In contrast, SS alleles of the poly(A) microsatellite had significantly elevated risks of EAC compared with SL/LL alleles. However, following permutation analyses to adjust for multiple comparisons, no significant associations were observed between any VDR gene polymorphism and EAC risk. Conclusions: VDR gene polymorphisms were not significantly associated with EAC development in this Irish population. Confirmation is required from larger studies.",

keywords = "Case-control studies, Esophageal adenocarcinoma, Polymorphism, Single nucleotide, Vitamin D receptor",

author = "Chang, {C. K.} and Mulholland, {H. G.} and Cantwell, {M. M.} and Anderson, {L. A.} and Johnston, {B. T.} and McKnight, {A. J.} and Thompson, {P. D.} and Watson, {R. G.P.} and Murray, {L. J.}",

note = "Acknowledgments The FINBAR study group members include LJ Murray (Queen{\textquoteright}s University Belfast), LA Anderson (Queen{\textquoteright}s University Belfast), BT Johnston (Belfast Health & Social Care Trust), RGP Watson (Belfast Health & Social Care Trust), J McGuigan (Belfast Health & Social Care Trust), HR Ferguson (Belfast Health & Social Care Trust), SJ Murphy (St Vincent{\textquoteright}s Hospital Dublin), JV Reynolds (St James{\textquoteright} Hospital, Dublin) and H Comber (National Cancer Registry of Ireland). We appreciate the contributions made by the study participants, their families and all who assisted with the study, particularly the Northern Ireland Cancer Registry and National Cancer Registry Cork. This research was supported by funding from the Ulster Cancer Foundation, the Northern Ireland R&D office and the Health Research Board.",

year = "2012",

doi = "10.1007/s12029-011-9322-9",

language = "English",

volume = "43",

pages = "512--517",

journal = "Journal of Gastrointestinal Cancer",

issn = "1941-6628",

publisher = "Humana Press",

}

TY - JOUR

T1 - Vitamin D receptor gene variants and esophageal adenocarcinoma risk

T2 - A population-based case-control study

AU - Chang, C. K.

AU - Mulholland, H. G.

AU - Cantwell, M. M.

AU - Anderson, L. A.

AU - Johnston, B. T.

AU - McKnight, A. J.

AU - Thompson, P. D.

AU - Watson, R. G.P.

AU - Murray, L. J.

N1 - Acknowledgments The FINBAR study group members include LJ Murray (Queen’s University Belfast), LA Anderson (Queen’s University Belfast), BT Johnston (Belfast Health & Social Care Trust), RGP Watson (Belfast Health & Social Care Trust), J McGuigan (Belfast Health & Social Care Trust), HR Ferguson (Belfast Health & Social Care Trust), SJ Murphy (St Vincent’s Hospital Dublin), JV Reynolds (St James’ Hospital, Dublin) and H Comber (National Cancer Registry of Ireland). We appreciate the contributions made by the study participants, their families and all who assisted with the study, particularly the Northern Ireland Cancer Registry and National Cancer Registry Cork. This research was supported by funding from the Ulster Cancer Foundation, the Northern Ireland R&D office and the Health Research Board.

PY - 2012

Y1 - 2012

N2 - Purpose: Polymorphisms in the vitamin D receptor (VDR) gene may be of etiological importance in determining cancer risk. The aim of this study was to assess the association between common VDR gene polymorphisms and esophageal adenocarcinoma (EAC) risk in an all-Ireland population-based case-control study. Methods: EAC cases and frequency-matched controls by age and gender recruited between March 2002 and December 2004 throughout Ireland were included. Participants were interviewed, and a blood sample collected for DNA extraction. Twenty-seven single nucleotide polymorphisms in the VDR gene were genotyped using Sequenom or TaqMan assays while the poly(A) microsatellite was genotyped by fluorescent fragment analysis. Unconditional logistic regression was applied to assess the association between VDR polymorphisms and EAC risk. Results: A total of 224 cases of EAC and 256 controls were involved in analyses. After adjustment for potential confounders, TT homozygotes at rs2238139 and rs2107301 had significantly reduced risks of EAC compared with CC homozygotes. In contrast, SS alleles of the poly(A) microsatellite had significantly elevated risks of EAC compared with SL/LL alleles. However, following permutation analyses to adjust for multiple comparisons, no significant associations were observed between any VDR gene polymorphism and EAC risk. Conclusions: VDR gene polymorphisms were not significantly associated with EAC development in this Irish population. Confirmation is required from larger studies.

AB - Purpose: Polymorphisms in the vitamin D receptor (VDR) gene may be of etiological importance in determining cancer risk. The aim of this study was to assess the association between common VDR gene polymorphisms and esophageal adenocarcinoma (EAC) risk in an all-Ireland population-based case-control study. Methods: EAC cases and frequency-matched controls by age and gender recruited between March 2002 and December 2004 throughout Ireland were included. Participants were interviewed, and a blood sample collected for DNA extraction. Twenty-seven single nucleotide polymorphisms in the VDR gene were genotyped using Sequenom or TaqMan assays while the poly(A) microsatellite was genotyped by fluorescent fragment analysis. Unconditional logistic regression was applied to assess the association between VDR polymorphisms and EAC risk. Results: A total of 224 cases of EAC and 256 controls were involved in analyses. After adjustment for potential confounders, TT homozygotes at rs2238139 and rs2107301 had significantly reduced risks of EAC compared with CC homozygotes. In contrast, SS alleles of the poly(A) microsatellite had significantly elevated risks of EAC compared with SL/LL alleles. However, following permutation analyses to adjust for multiple comparisons, no significant associations were observed between any VDR gene polymorphism and EAC risk. Conclusions: VDR gene polymorphisms were not significantly associated with EAC development in this Irish population. Confirmation is required from larger studies.

KW - Case-control studies

KW - Esophageal adenocarcinoma

KW - Polymorphism

KW - Single nucleotide

KW - Vitamin D receptor

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DO - 10.1007/s12029-011-9322-9

M3 - Article

C2 - 21948293

AN - SCOPUS:84866386644

SN - 1941-6628

VL - 43

SP - 512

EP - 517

JO - Journal of Gastrointestinal Cancer

JF - Journal of Gastrointestinal Cancer

ER -

Vitamin D receptor gene variants and esophageal adenocarcinoma risk: A population-based case-control study

Abstract

Bibliographical note

Keywords

UN SDGs

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