Vitamin-E requirements, transport, and metabolism: role of alpha-tocopherol-binding proteins

A K Duttaroy, M J Gordon, F M Campbell, G G Duthie, W P T James

Research output: Contribution to journalLiterature review

72 Citations (Scopus)

Abstract

Vitamin E (RRR-alpha-tocapherol) is a lipid-soluble antioxidant that is present in the membranes of intracellular organelles. There it plays an important role in the suppression of free radical-induced lipid peroxidation. There are eight naturally occurring homologues of vitamin E that differ in their structure and in biological activity in vivo and in vitro. Although gamma-tocopherol is a more effective free radical scavenger than alpha-tocopherol in vitro, the reverse is true in vivo, suggesting that the tocopherol distribution systems favor the localization of alpha-tocopherol at the sites where if is required. Vitamin E is transported in plasma primarily by lipoproteins, but little is known of how it is transported intracellularly. A 30 kDa alpha-tocopherol-binding protein in the liver cytoplasm may regulate plasma vitamin E concentrations by preferentially incorporating the vitamin E homologue, RRR-alpha-tocopherol (alpha-tocopherol), into nascent very low density lipoproteins. However this alpha-tocopherol-binding protein is unique to the hepatocyte, whereas alpha-tocopherol is present in the cells of all major tissues. Moreover alpha-tocopherol accumulates at those sires within the cell where oxygen radical production is greatest and thus where it is most required; in the membranes of heavy mitochondria, light mitochondria, and endoplasmic reticulum. This raises the question of how the lipid-soluble alpha-tocopherol is transported intracellularly in different tissues. We have identified a new alpha-tocopherol-binding protein of molecular mass 14.2 kDa in the cytosol of heart and liver This protein specifically binds alpha-tocopherol in preference to the delta- and gamma-homologues but does not bind oleate. Studies on immunoreactivity and ligand specificity of the protein suggest that it is not a fatty acid-binding protein. The 14.2 kDa alpha-tocopherol-binding protein stimulates the transfer of alpha-tocopherol from liposomes to mitochondria in vitro by 8 to 10 fold. We suggest that this low molecular mass TBP may be responsible for the intracellular transport and distribution of alpha-tocopherol in the tissues.

Original languageEnglish
Pages (from-to)562-570
Number of pages9
JournalThe Journal of Nutritional Biochemistry
Volume5
Issue number12
DOIs
Publication statusPublished - Dec 1994

Keywords

  • vitamin-E (RRR-alpha-tocopherol)
  • alpha-tocopherol-binding protein (TBP)
  • plasma membrane alpha-tocopherol-binding protein (TBPPM)
  • vitamin E transport
  • lipoproteins (VLDL, HDL, and LDL)
  • fatty Acid-binding protein (FABP)
  • low-density-lipoprotein
  • kinase-c activity
  • ischemic-heart-disease
  • rat-liver
  • gamma-tocopherol
  • e-deficiency
  • intestinal-absorption
  • lipid-peroxidation
  • e consumption
  • fatty-acids

Cite this

Vitamin-E requirements, transport, and metabolism : role of alpha-tocopherol-binding proteins. / Duttaroy, A K; Gordon, M J; Campbell, F M; Duthie, G G; James, W P T.

In: The Journal of Nutritional Biochemistry, Vol. 5, No. 12, 12.1994, p. 562-570.

Research output: Contribution to journalLiterature review

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T2 - role of alpha-tocopherol-binding proteins

AU - Duttaroy, A K

AU - Gordon, M J

AU - Campbell, F M

AU - Duthie, G G

AU - James, W P T

PY - 1994/12

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N2 - Vitamin E (RRR-alpha-tocapherol) is a lipid-soluble antioxidant that is present in the membranes of intracellular organelles. There it plays an important role in the suppression of free radical-induced lipid peroxidation. There are eight naturally occurring homologues of vitamin E that differ in their structure and in biological activity in vivo and in vitro. Although gamma-tocopherol is a more effective free radical scavenger than alpha-tocopherol in vitro, the reverse is true in vivo, suggesting that the tocopherol distribution systems favor the localization of alpha-tocopherol at the sites where if is required. Vitamin E is transported in plasma primarily by lipoproteins, but little is known of how it is transported intracellularly. A 30 kDa alpha-tocopherol-binding protein in the liver cytoplasm may regulate plasma vitamin E concentrations by preferentially incorporating the vitamin E homologue, RRR-alpha-tocopherol (alpha-tocopherol), into nascent very low density lipoproteins. However this alpha-tocopherol-binding protein is unique to the hepatocyte, whereas alpha-tocopherol is present in the cells of all major tissues. Moreover alpha-tocopherol accumulates at those sires within the cell where oxygen radical production is greatest and thus where it is most required; in the membranes of heavy mitochondria, light mitochondria, and endoplasmic reticulum. This raises the question of how the lipid-soluble alpha-tocopherol is transported intracellularly in different tissues. We have identified a new alpha-tocopherol-binding protein of molecular mass 14.2 kDa in the cytosol of heart and liver This protein specifically binds alpha-tocopherol in preference to the delta- and gamma-homologues but does not bind oleate. Studies on immunoreactivity and ligand specificity of the protein suggest that it is not a fatty acid-binding protein. The 14.2 kDa alpha-tocopherol-binding protein stimulates the transfer of alpha-tocopherol from liposomes to mitochondria in vitro by 8 to 10 fold. We suggest that this low molecular mass TBP may be responsible for the intracellular transport and distribution of alpha-tocopherol in the tissues.

AB - Vitamin E (RRR-alpha-tocapherol) is a lipid-soluble antioxidant that is present in the membranes of intracellular organelles. There it plays an important role in the suppression of free radical-induced lipid peroxidation. There are eight naturally occurring homologues of vitamin E that differ in their structure and in biological activity in vivo and in vitro. Although gamma-tocopherol is a more effective free radical scavenger than alpha-tocopherol in vitro, the reverse is true in vivo, suggesting that the tocopherol distribution systems favor the localization of alpha-tocopherol at the sites where if is required. Vitamin E is transported in plasma primarily by lipoproteins, but little is known of how it is transported intracellularly. A 30 kDa alpha-tocopherol-binding protein in the liver cytoplasm may regulate plasma vitamin E concentrations by preferentially incorporating the vitamin E homologue, RRR-alpha-tocopherol (alpha-tocopherol), into nascent very low density lipoproteins. However this alpha-tocopherol-binding protein is unique to the hepatocyte, whereas alpha-tocopherol is present in the cells of all major tissues. Moreover alpha-tocopherol accumulates at those sires within the cell where oxygen radical production is greatest and thus where it is most required; in the membranes of heavy mitochondria, light mitochondria, and endoplasmic reticulum. This raises the question of how the lipid-soluble alpha-tocopherol is transported intracellularly in different tissues. We have identified a new alpha-tocopherol-binding protein of molecular mass 14.2 kDa in the cytosol of heart and liver This protein specifically binds alpha-tocopherol in preference to the delta- and gamma-homologues but does not bind oleate. Studies on immunoreactivity and ligand specificity of the protein suggest that it is not a fatty acid-binding protein. The 14.2 kDa alpha-tocopherol-binding protein stimulates the transfer of alpha-tocopherol from liposomes to mitochondria in vitro by 8 to 10 fold. We suggest that this low molecular mass TBP may be responsible for the intracellular transport and distribution of alpha-tocopherol in the tissues.

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KW - lipoproteins (VLDL, HDL, and LDL)

KW - fatty Acid-binding protein (FABP)

KW - low-density-lipoprotein

KW - kinase-c activity

KW - ischemic-heart-disease

KW - rat-liver

KW - gamma-tocopherol

KW - e-deficiency

KW - intestinal-absorption

KW - lipid-peroxidation

KW - e consumption

KW - fatty-acids

U2 - 10.1016/0955-2863(94)90010-8

DO - 10.1016/0955-2863(94)90010-8

M3 - Literature review

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EP - 570

JO - The Journal of Nutritional Biochemistry

JF - The Journal of Nutritional Biochemistry

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