Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

Gordon T.A. McEwan; J Hunter; B H Hirst; N L Simmons

Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

Gordon T.A. McEwan, J Hunter, B H Hirst, N L Simmons

Research output: Contribution to journal › Article › peer-review

Abstract

The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.

Original language	English
Pages (from-to)	233-6
Number of pages	4
Journal	FEBS Letters
Volume	304
Issue number	2-3
Publication status	Published - 15 Jun 1992

Keywords

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Biological Transport
Chloride Channels
Chlorides
Colforsin
Humans
Intestinal Mucosa
Membrane Glycoproteins
Membrane Proteins
P-Glycoprotein
Tumor Cells, Cultured
Vinblastine

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@article{2831c105fff9470ebd6499c6064b279b,

title = "Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers",

abstract = "The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.",

keywords = "4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, Biological Transport, Chloride Channels, Chlorides, Colforsin, Humans, Intestinal Mucosa, Membrane Glycoproteins, Membrane Proteins, P-Glycoprotein, Tumor Cells, Cultured, Vinblastine",

author = "McEwan, {Gordon T.A.} and J Hunter and Hirst, {B H} and Simmons, {N L}",

year = "1992",

month = jun,

day = "15",

language = "English",

volume = "304",

pages = "233--6",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "2-3",

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TY - JOUR

T1 - Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

AU - McEwan, Gordon T.A.

AU - Hunter, J

AU - Hirst, B H

AU - Simmons, N L

PY - 1992/6/15

Y1 - 1992/6/15

N2 - The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.

AB - The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.

KW - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid

KW - 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid

KW - Biological Transport

KW - Chloride Channels

KW - Chlorides

KW - Colforsin

KW - Humans

KW - Intestinal Mucosa

KW - Membrane Glycoproteins

KW - Membrane Proteins

KW - P-Glycoprotein

KW - Tumor Cells, Cultured

KW - Vinblastine

M3 - Article

C2 - 1352259

SN - 0014-5793

VL - 304

SP - 233

EP - 236

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

Abstract

Keywords

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