Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

G T McEwan, J Hunter, B H Hirst, N L Simmons

Research output: Contribution to journalArticle

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Abstract

The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.
Original languageEnglish
Pages (from-to)233-6
Number of pages4
JournalFEBS Letters
Volume304
Issue number2-3
Publication statusPublished - 15 Jun 1992

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Vinblastine
P-Glycoprotein
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Colforsin
Swelling
Adenocarcinoma
Fluxes

Keywords

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
  • Biological Transport
  • Chloride Channels
  • Chlorides
  • Colforsin
  • Humans
  • Intestinal Mucosa
  • Membrane Glycoproteins
  • Membrane Proteins
  • P-Glycoprotein
  • Tumor Cells, Cultured
  • Vinblastine

Cite this

Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers. / McEwan, G T; Hunter, J; Hirst, B H; Simmons, N L.

In: FEBS Letters, Vol. 304, No. 2-3, 15.06.1992, p. 233-6.

Research output: Contribution to journalArticle

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abstract = "The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.",
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T1 - Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

AU - McEwan, G T

AU - Hunter, J

AU - Hirst, B H

AU - Simmons, N L

PY - 1992/6/15

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N2 - The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.

AB - The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.

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KW - 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid

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KW - Chloride Channels

KW - Chlorides

KW - Colforsin

KW - Humans

KW - Intestinal Mucosa

KW - Membrane Glycoproteins

KW - Membrane Proteins

KW - P-Glycoprotein

KW - Tumor Cells, Cultured

KW - Vinblastine

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JO - FEBS Letters

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SN - 0014-5793

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