What's the Catch? Archaeological application of rapid collagen-based species identification for Pacific Salmon

K Korzow-Richter, K McGrath, Edouard Masson-MacLean, S Hickinbotham, A Tedder, Kate Britton, Z Bottomley, Keith Dobney, A Hulme-Beaman, M Zona, R Fischer, MJ Collins, C Speller* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pacific salmon (Oncorhynchus spp.) are ecological and cultural keystone species along the Northwest Coast of North America and are ubiquitous in archaeological sites of the region. The inability to morphologically identify salmonid post-cranial remains to species, however, can limit our understanding of the ecological and cultural role different taxa played in the seasonal subsistence practices of Indigenous groups in the past. Here, we present a rapid, cost-effective ZooMS method to distinguish salmonid species based on collagen peptide mass-fingerprinting. Using modern reference material and an assemblage of 28 DNA-identified salmonid bones from the pre-contact Yup'ik site of Nunalleq, Western Alaska, we apply high-resolution mass spectrometry (LC-MS/MS) to identify a series of potential collagen peptide markers to distinguish Pacific salmon. We then confirm these peptide markers with a blind ZooMS analysis (MALDI-TOF-MS) of the archaeological remains. We successfully distinguish five species of anadmmous salmon with this ZooMS approach, including one specimen that could not be identified through ancient DNA analysis. Our biomolecular identification of chum (43%), sockeye (21%), chinook (18%), coho (11%) and pink (7%), confirm the exploitation of all five available species of salmonid at Nunalleq.

Original languageEnglish
Article number105116
Number of pages16
JournalJournal of Archaeological Science
Volume116
Early online date4 Mar 2020
DOIs
Publication statusPublished - Apr 2020

Keywords

  • ZooMS
  • collagen peptide mass fingerprinting
  • Pacific salmon
  • Yup'Ik
  • Nunalleq
  • RAINBOW-TROUT
  • MITOCHONDRIAL GENOME
  • ONCORHYNCHUS SPP.
  • ANCIENT DNA
  • AGE-DETERMINATION
  • YUKON-KUSKOKWIM DELTA
  • GENOME DUPLICATION
  • CHINOOK SALMON
  • Collagen peptide mass fingerprinting
  • Pacific Salmon
  • DIONISIO POINT
  • NORTH-AMERICA

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    Korzow-Richter, K., McGrath, K., Masson-MacLean, E., Hickinbotham, S., Tedder, A., Britton, K., Bottomley, Z., Dobney, K., Hulme-Beaman, A., Zona, M., Fischer, R., Collins, MJ., & Speller, C. (2020). What's the Catch? Archaeological application of rapid collagen-based species identification for Pacific Salmon. Journal of Archaeological Science, 116, [105116]. https://doi.org/10.1016/j.jas.2020.105116