Abstract
Dermacoccus abyssi strain MT1.1T is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.
Original language | English |
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Article number | 131 |
Number of pages | 18 |
Journal | Marine Drugs |
Volume | 18 |
Issue number | 3 |
DOIs | |
Publication status | Published - 25 Feb 2020 |
Bibliographical note
Funding:This work was supported by Researchers Supporting Project number (RSP-2019/132), King Saud University, Riyadh, Saudi Arabia.
Acknowledgements:
A.S.A. is thankful to the Researchers Supporting Project number (RSP-2019/132), King Saud University, Riyadh, Saudi Arabia.The authors are also indebted to Wasu Pathom-Aree (Chiang Mai University, Thailand) who isolated the strain.
Conflicts of Interest:
The authors declare that they don’t have any conflicts of interest.
Author Contributions:
W.M.A.-M., B.L. and A.V.K. were responsible for the whole genome sequencing and bioinformatic analyses; W.M.A.-M. and A.S.A. analyzed the sequence data for protein coding genes and their functions; B.J. and M.J. were responsible for cultivating the organism and for the isolation and identification of dermacozine M; I.N. and M.G. isolated and purified the DNA for whole genome sequencing and was responsible for the systematics; D.P.-P. and J.R. did the computational calculations; J.N.T. did the 13C chemical shift simulation and W.M.A.-M., B.J., B.L., A.S.A., M.G., D.P.-P., M.G., M.J. and A.V.K. prepared the manuscript; all of authors read and ap
Keywords
- Mariana Trench
- Dermacoccus abyssi MT1.1T
- dermacozines
- genome sequencing
- biosynthetic gene clusters
- Dermacozines
- Genome sequencing
- Biosynthetic gene clusters
- Dermacoccus abyssi MT1.1
- Dermacoccus abyssi MT1
- COLD SHOCK
- BACTERIA
- SP NOV.
- GENES
- SHEWANELLA-VIOLACEA
- 1(T)
- STRAIN
- DERMACOZINES