Whole Genome Sequence of Dermacoccus abyssi MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors

Wael M Abdel-Mageed* (Corresponding Author), Bertalan Juhasz, Burhan Lehri, Ali S Alqahtani, Imen Nouioui, Dawrin Pech-Puch, Jioji N Tabudravu, Michael Goodfellow, Jaime Rodríguez, Marcel Jaspars, Andrey V Karlyshev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
7 Downloads (Pure)

Abstract

Dermacoccus abyssi strain MT1.1T is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.

Original languageEnglish
Article number131
Number of pages18
JournalMarine Drugs
Volume18
Issue number3
DOIs
Publication statusPublished - 25 Feb 2020

Bibliographical note

Funding:
This work was supported by Researchers Supporting Project number (RSP-2019/132), King Saud University, Riyadh, Saudi Arabia.

Acknowledgements:
A.S.A. is thankful to the Researchers Supporting Project number (RSP-2019/132), King Saud University, Riyadh, Saudi Arabia.The authors are also indebted to Wasu Pathom-Aree (Chiang Mai University, Thailand) who isolated the strain.

Conflicts of Interest:
The authors declare that they don’t have any conflicts of interest.

Author Contributions:
W.M.A.-M., B.L. and A.V.K. were responsible for the whole genome sequencing and bioinformatic analyses; W.M.A.-M. and A.S.A. analyzed the sequence data for protein coding genes and their functions; B.J. and M.J. were responsible for cultivating the organism and for the isolation and identification of dermacozine M; I.N. and M.G. isolated and purified the DNA for whole genome sequencing and was responsible for the systematics; D.P.-P. and J.R. did the computational calculations; J.N.T. did the 13C chemical shift simulation and W.M.A.-M., B.J., B.L., A.S.A., M.G., D.P.-P., M.G., M.J. and A.V.K. prepared the manuscript; all of authors read and ap

Keywords

  • Mariana Trench
  • Dermacoccus abyssi MT1.1T
  • dermacozines
  • genome sequencing
  • biosynthetic gene clusters
  • Dermacozines
  • Genome sequencing
  • Biosynthetic gene clusters
  • Dermacoccus abyssi MT1.1
  • Dermacoccus abyssi MT1
  • COLD SHOCK
  • BACTERIA
  • SP NOV.
  • GENES
  • SHEWANELLA-VIOLACEA
  • 1(T)
  • STRAIN
  • DERMACOZINES

Fingerprint

Dive into the research topics of 'Whole Genome Sequence of Dermacoccus abyssi MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors'. Together they form a unique fingerprint.

Cite this