@article{c6516c93346444c18168a3050ee48575,
title = "Whole Genome Sequence of Dermacoccus abyssi MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors",
abstract = "Dermacoccus abyssi strain MT1.1T is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.",
keywords = "Mariana Trench, Dermacoccus abyssi MT1.1T, dermacozines, genome sequencing, biosynthetic gene clusters, Dermacozines, Genome sequencing, Biosynthetic gene clusters, Dermacoccus abyssi MT1.1, Dermacoccus abyssi MT1, COLD SHOCK, BACTERIA, SP NOV., GENES, SHEWANELLA-VIOLACEA, 1(T), STRAIN, DERMACOZINES",
author = "Abdel-Mageed, {Wael M} and Bertalan Juhasz and Burhan Lehri and Alqahtani, {Ali S} and Imen Nouioui and Dawrin Pech-Puch and Tabudravu, {Jioji N} and Michael Goodfellow and Jaime Rodr{\'i}guez and Marcel Jaspars and Karlyshev, {Andrey V}",
note = "Funding: This work was supported by Researchers Supporting Project number (RSP-2019/132), King Saud University, Riyadh, Saudi Arabia. Acknowledgements: A.S.A. is thankful to the Researchers Supporting Project number (RSP-2019/132), King Saud University, Riyadh, Saudi Arabia.The authors are also indebted to Wasu Pathom-Aree (Chiang Mai University, Thailand) who isolated the strain. Conflicts of Interest: The authors declare that they don{\textquoteright}t have any conflicts of interest. Author Contributions: W.M.A.-M., B.L. and A.V.K. were responsible for the whole genome sequencing and bioinformatic analyses; W.M.A.-M. and A.S.A. analyzed the sequence data for protein coding genes and their functions; B.J. and M.J. were responsible for cultivating the organism and for the isolation and identification of dermacozine M; I.N. and M.G. isolated and purified the DNA for whole genome sequencing and was responsible for the systematics; D.P.-P. and J.R. did the computational calculations; J.N.T. did the 13C chemical shift simulation and W.M.A.-M., B.J., B.L., A.S.A., M.G., D.P.-P., M.G., M.J. and A.V.K. prepared the manuscript; all of authors read and ap",
year = "2020",
month = feb,
day = "25",
doi = "10.3390/md18030131",
language = "English",
volume = "18",
journal = "Marine Drugs",
issn = "1660-3397",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "3",
}