Whole Genome Sequence of Dermacoccus abyssi MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors

Wael M Abdel-Mageed* (Corresponding Author), Bertalan Juhasz, Burhan Lehri, Ali S Alqahtani, Imen Nouioui, Dawrin Pech-Puch, Jioji N Tabudravu, Michael Goodfellow, Jaime Rodríguez, Marcel Jaspars, Andrey V Karlyshev

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

Dermacoccus abyssi strain MT1.1T is a piezotolerant actinobacterium that was isolated from Mariana Trench sediment collected at a depth of 10898 m. The organism was found to produce ten dermacozines (A‒J) that belonged to a new phenazine family and which displayed various biological activities such as radical scavenging and cytotoxicity. Here, we report on the isolation and identification of a new dermacozine compound, dermacozine M, the chemical structure of which was determined using 1D and 2D-NMR, and high resolution MS. A whole genome sequence of the strain contained six secondary metabolite-biosynthetic gene clusters (BGCs), including one responsible for the biosynthesis of a family of phenazine compounds. A pathway leading to the biosynthesis of dermacozines is proposed. Bioinformatic analyses of key stress-related genes provide an insight into how the organism adapted to the environmental conditions that prevail in the deep-sea.

Original languageEnglish
Article number131
Number of pages18
JournalMarine Drugs
Volume18
Issue number3
DOIs
Publication statusPublished - 25 Feb 2020

Keywords

  • Mariana Trench
  • Dermacoccus abyssi MT1.1T
  • dermacozines
  • genome sequencing
  • biosynthetic gene clusters
  • Dermacozines
  • Genome sequencing
  • Biosynthetic gene clusters
  • Dermacoccus abyssi MT1.1
  • Dermacoccus abyssi MT1
  • COLD SHOCK
  • BACTERIA
  • SP NOV.
  • GENES
  • SHEWANELLA-VIOLACEA
  • 1(T)
  • STRAIN
  • DERMACOZINES

ASJC Scopus subject areas

  • Drug Discovery

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    Abdel-Mageed, W. M., Juhasz, B., Lehri, B., Alqahtani, A. S., Nouioui, I., Pech-Puch, D., Tabudravu, J. N., Goodfellow, M., Rodríguez, J., Jaspars, M., & Karlyshev, A. V. (2020). Whole Genome Sequence of Dermacoccus abyssi MT1.1 Isolated from the Challenger Deep of the Mariana Trench Reveals Phenazine Biosynthesis Locus and Environmental Adaptation Factors. Marine Drugs, 18(3), [131]. https://doi.org/10.3390/md18030131