Why two are not enough: degradation of p-toluenesulfonate by a bacterial community from a pristine site in Moorea, French Polynesia

Tewes Tralau, Eun Chan Yang, Carola Tralau, Alasdair M Cook, Frithjof C Küpper, Frithjof Christian Kuepper

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In previous work, only one culture (strain TA12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (TSA) as a sole source of carbon and energy for aerobic growth. 'Strain TA12' has now been recognized as a community of three bacteria: Achromobacter xylosoxidans TA12-A, Ensifer adhaerens TA12-B and Pseudomonas nitroreducens TA12-C. Achromobacter xylosoxidans TA12-A and E. adhaerens TA12-B were identified as the TSA degraders. These two organisms contain several tsa genes from the Tntsa cluster described previously in Comamonas testosteroni T-2 and use the tsa pathway. Apparently, due to vitamin auxotrophy, the growth of the pure cultures with TSA was markedly slower than the growth of the community with TSA. The third bacterium (P. nitroreducens) TA12-C is, then, a provider of essential vitamins for the TSA degraders and occurs at a low frequency.
Original languageEnglish
Pages (from-to)123-9
Number of pages7
JournalFEMS Microbiology Letters
Volume316
Issue number2
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Polynesia
Achromobacter denitrificans
Vitamins
Comamonas testosteroni
Growth
Bacteria
Xenobiotics
Multigene Family
Pseudomonas
Carbon
4-toluenesulfonic acid

Keywords

  • achromobacter denitrificans
  • bacterial Proteins
  • benzenesulfonates
  • biodegradation, environmental
  • molecular sequence data
  • Polynesia
  • pseudomonas
  • rhizobiaceae
  • xenobiotics

Cite this

Why two are not enough : degradation of p-toluenesulfonate by a bacterial community from a pristine site in Moorea, French Polynesia. / Tralau, Tewes; Yang, Eun Chan; Tralau, Carola; Cook, Alasdair M; Küpper, Frithjof C; Kuepper, Frithjof Christian.

In: FEMS Microbiology Letters, Vol. 316, No. 2, 03.2011, p. 123-9.

Research output: Contribution to journalArticle

Tralau, Tewes ; Yang, Eun Chan ; Tralau, Carola ; Cook, Alasdair M ; Küpper, Frithjof C ; Kuepper, Frithjof Christian. / Why two are not enough : degradation of p-toluenesulfonate by a bacterial community from a pristine site in Moorea, French Polynesia. In: FEMS Microbiology Letters. 2011 ; Vol. 316, No. 2. pp. 123-9.
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abstract = "In previous work, only one culture (strain TA12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (TSA) as a sole source of carbon and energy for aerobic growth. 'Strain TA12' has now been recognized as a community of three bacteria: Achromobacter xylosoxidans TA12-A, Ensifer adhaerens TA12-B and Pseudomonas nitroreducens TA12-C. Achromobacter xylosoxidans TA12-A and E. adhaerens TA12-B were identified as the TSA degraders. These two organisms contain several tsa genes from the Tntsa cluster described previously in Comamonas testosteroni T-2 and use the tsa pathway. Apparently, due to vitamin auxotrophy, the growth of the pure cultures with TSA was markedly slower than the growth of the community with TSA. The third bacterium (P. nitroreducens) TA12-C is, then, a provider of essential vitamins for the TSA degraders and occurs at a low frequency.",
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