Wound healing in rat cornea: the role of electric currents

Brian Reid, Bing Song, C. McCaig, Min Zhao

    Research output: Contribution to journalArticle

    101 Citations (Scopus)

    Abstract

    Human corneal epithelial cells respond rapidly following injury to restore the integrity of the ocular surface. What stimulates and guides cells to move into the wound to heal? One candidate is the wound-induced electric field. Using vibrating probe techniques, we provide detailed temporal and spatial mapping of endogenous electric currents at rat corneal wounds. We find Cl- and Na+ are the major components of electric currents in rat corneal wounds. Na+ is the major component of ionic transport in the resting (nonwounded) rat cornea and of the wound center leakage current, whereas Cl- is a more important component of the endogenous electrical current at the wound edges. Enhancing or decreasing Cl- flow with clinically approved pharmacological agents such as aminophylline, ascorbic acid, or furosemide increased or decreased endogenous wound electric currents, respectively. These changes in wound currents correlated directly with the rate of wound healing in vivo. Thus, pharmacologically enhancing or decreasing wound-induced electric currents increased and decreased wound healing rate, respectively. This may have wide-reaching and novel therapeutic potential in the management of wound healing and may help explain some mechanistic aspects of the effects of some clinically used agents.

    Original languageEnglish
    Pages (from-to)379-386
    Number of pages7
    JournalThe FASEB Journal
    Volume19
    Issue number3
    DOIs
    Publication statusPublished - Mar 2005

    Keywords

    • cornea epithelium
    • vibrating probe
    • electric field
    • CHLORIDE TRANSPORT
    • DIRECTED MIGRATION
    • EPITHELIAL-CELLS
    • FROG CORNEA
    • FIELDS
    • STIMULATION
    • SODIUM
    • REGENERATION
    • ORIENTATION
    • INHIBITION

    Cite this