The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India

Prasanth Manohar, Thamaraiselvan Shanthini, Ramankannan Ayyanar, Bulent Bozdogan, Aruni Wilson, Ashok J Tamhankar, Ramesh Nachimuthu, Bruno S Lopes

Research output: Contribution to journalArticle

12 Citations (Scopus)
10 Downloads (Pure)

Abstract

Purpose. The occurrence of carbapenem- and colistin-resistance among Gram-negative bacteria is increasing worldwide. The aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in Tamil Nadu, India.

Methodology. The clinical isolates (n=89) used in this study were collected from two diagnostic centres in Tamil Nadu, India. The bacterial isolates were screened for meropenem- and colistin-resistance. Further, resistance genes bla NDM-1, bla OXA-48-like, bla IMP, bla VIM, bla KPC, mcr-1 and mcr-2 and integrons were studied. The synergistic effect of meropenem in combination with colistin was assessed.

Results. A total of 89 bacterial isolates were studied which included Escherichia coli (n=43), Klebsiella pneumoniae (n=18), Pseudomonas aeruginosa (n=10), Enterobacter cloacae (n=6), Acinetobacter baumannii (n=5), Klebsiella oxytoca (n=4), Proteus mirabilis (n=2) and Salmonella paratyphi (n=1). MIC testing showed that 58/89 (65 %) and 29/89 (32 %) isolates were resistant to meropenem and colistin, respectively, whereas 27/89 (30 %) isolates were resistant to both antibiotics. Escherichia coli, K. pneumoniae, K. oxytoca, Pseudomonas aeruginosa, and Enterobacter cloacae isolates were bla NDM-1-positive (n=20). Some strains of Escherichia coli, K. pneumoniae and K. oxytoca were bla OXA-181-positive (n=4). Class 1, 2 and 3 integrons were found in 24, 20 and 3 isolates, respectively. Nine NDM-1-positive Escherichia coli strains could transfer carbapenem resistance via plasmids to susceptible Escherichia coli AB1157. Meropenem and colistin showed synergy in 10/20 (50 %) isolates by 24 h time-kill studies.

Conclusion. Our results highlight the distribution of carbapenem- and colistin-resistance in Gram-negative bacteria isolated from the Tamil Nadu region in South India.
Original languageEnglish
Pages (from-to)874-883
Number of pages9
JournalJournal of Medical Microbiology
Volume66
Early online date3 Jul 2017
DOIs
Publication statusPublished - Jul 2017

Fingerprint

Colistin
meropenem
Carbapenems
Gram-Negative Bacteria
India
Klebsiella oxytoca
Escherichia coli
Klebsiella pneumoniae
Integrons
Enterobacter cloacae
Pseudomonas aeruginosa
Salmonella paratyphi A
Acinetobacter baumannii
Inosine Monophosphate
Proteus mirabilis
Plasmids
Anti-Bacterial Agents

Keywords

  • integron
  • plasmid-bound resistance
  • transferability
  • New Delhi metallo-beta lactamases
  • carbapenemases

Cite this

The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India. / Manohar, Prasanth ; Shanthini, Thamaraiselvan ; Ayyanar, Ramankannan ; Bozdogan, Bulent ; Wilson, Aruni ; Tamhankar, Ashok J ; Nachimuthu, Ramesh ; Lopes, Bruno S.

In: Journal of Medical Microbiology, Vol. 66, 07.2017, p. 874-883.

Research output: Contribution to journalArticle

Manohar, Prasanth ; Shanthini, Thamaraiselvan ; Ayyanar, Ramankannan ; Bozdogan, Bulent ; Wilson, Aruni ; Tamhankar, Ashok J ; Nachimuthu, Ramesh ; Lopes, Bruno S. / The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India. In: Journal of Medical Microbiology. 2017 ; Vol. 66. pp. 874-883.
@article{46e9e983ba4b4cfb930ee22a30228214,
title = "The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India",
abstract = "Purpose. The occurrence of carbapenem- and colistin-resistance among Gram-negative bacteria is increasing worldwide. The aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in Tamil Nadu, India.Methodology. The clinical isolates (n=89) used in this study were collected from two diagnostic centres in Tamil Nadu, India. The bacterial isolates were screened for meropenem- and colistin-resistance. Further, resistance genes bla NDM-1, bla OXA-48-like, bla IMP, bla VIM, bla KPC, mcr-1 and mcr-2 and integrons were studied. The synergistic effect of meropenem in combination with colistin was assessed.Results. A total of 89 bacterial isolates were studied which included Escherichia coli (n=43), Klebsiella pneumoniae (n=18), Pseudomonas aeruginosa (n=10), Enterobacter cloacae (n=6), Acinetobacter baumannii (n=5), Klebsiella oxytoca (n=4), Proteus mirabilis (n=2) and Salmonella paratyphi (n=1). MIC testing showed that 58/89 (65 {\%}) and 29/89 (32 {\%}) isolates were resistant to meropenem and colistin, respectively, whereas 27/89 (30 {\%}) isolates were resistant to both antibiotics. Escherichia coli, K. pneumoniae, K. oxytoca, Pseudomonas aeruginosa, and Enterobacter cloacae isolates were bla NDM-1-positive (n=20). Some strains of Escherichia coli, K. pneumoniae and K. oxytoca were bla OXA-181-positive (n=4). Class 1, 2 and 3 integrons were found in 24, 20 and 3 isolates, respectively. Nine NDM-1-positive Escherichia coli strains could transfer carbapenem resistance via plasmids to susceptible Escherichia coli AB1157. Meropenem and colistin showed synergy in 10/20 (50 {\%}) isolates by 24 h time-kill studies.Conclusion. Our results highlight the distribution of carbapenem- and colistin-resistance in Gram-negative bacteria isolated from the Tamil Nadu region in South India.",
keywords = "integron, plasmid-bound resistance, transferability, New Delhi metallo-beta lactamases, carbapenemases",
author = "Prasanth Manohar and Thamaraiselvan Shanthini and Ramankannan Ayyanar and Bulent Bozdogan and Aruni Wilson and Tamhankar, {Ashok J} and Ramesh Nachimuthu and Lopes, {Bruno S}",
note = "The authors would like to thank VIT University for providing research facilities. This study was financially supported by DST-SERB, Govt. of India, New Delhi, Ref: No. SERB/LS-930/2012.",
year = "2017",
month = "7",
doi = "10.1099/jmm.0.000508",
language = "English",
volume = "66",
pages = "874--883",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",

}

TY - JOUR

T1 - The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India

AU - Manohar, Prasanth

AU - Shanthini, Thamaraiselvan

AU - Ayyanar, Ramankannan

AU - Bozdogan, Bulent

AU - Wilson, Aruni

AU - Tamhankar, Ashok J

AU - Nachimuthu, Ramesh

AU - Lopes, Bruno S

N1 - The authors would like to thank VIT University for providing research facilities. This study was financially supported by DST-SERB, Govt. of India, New Delhi, Ref: No. SERB/LS-930/2012.

PY - 2017/7

Y1 - 2017/7

N2 - Purpose. The occurrence of carbapenem- and colistin-resistance among Gram-negative bacteria is increasing worldwide. The aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in Tamil Nadu, India.Methodology. The clinical isolates (n=89) used in this study were collected from two diagnostic centres in Tamil Nadu, India. The bacterial isolates were screened for meropenem- and colistin-resistance. Further, resistance genes bla NDM-1, bla OXA-48-like, bla IMP, bla VIM, bla KPC, mcr-1 and mcr-2 and integrons were studied. The synergistic effect of meropenem in combination with colistin was assessed.Results. A total of 89 bacterial isolates were studied which included Escherichia coli (n=43), Klebsiella pneumoniae (n=18), Pseudomonas aeruginosa (n=10), Enterobacter cloacae (n=6), Acinetobacter baumannii (n=5), Klebsiella oxytoca (n=4), Proteus mirabilis (n=2) and Salmonella paratyphi (n=1). MIC testing showed that 58/89 (65 %) and 29/89 (32 %) isolates were resistant to meropenem and colistin, respectively, whereas 27/89 (30 %) isolates were resistant to both antibiotics. Escherichia coli, K. pneumoniae, K. oxytoca, Pseudomonas aeruginosa, and Enterobacter cloacae isolates were bla NDM-1-positive (n=20). Some strains of Escherichia coli, K. pneumoniae and K. oxytoca were bla OXA-181-positive (n=4). Class 1, 2 and 3 integrons were found in 24, 20 and 3 isolates, respectively. Nine NDM-1-positive Escherichia coli strains could transfer carbapenem resistance via plasmids to susceptible Escherichia coli AB1157. Meropenem and colistin showed synergy in 10/20 (50 %) isolates by 24 h time-kill studies.Conclusion. Our results highlight the distribution of carbapenem- and colistin-resistance in Gram-negative bacteria isolated from the Tamil Nadu region in South India.

AB - Purpose. The occurrence of carbapenem- and colistin-resistance among Gram-negative bacteria is increasing worldwide. The aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in Tamil Nadu, India.Methodology. The clinical isolates (n=89) used in this study were collected from two diagnostic centres in Tamil Nadu, India. The bacterial isolates were screened for meropenem- and colistin-resistance. Further, resistance genes bla NDM-1, bla OXA-48-like, bla IMP, bla VIM, bla KPC, mcr-1 and mcr-2 and integrons were studied. The synergistic effect of meropenem in combination with colistin was assessed.Results. A total of 89 bacterial isolates were studied which included Escherichia coli (n=43), Klebsiella pneumoniae (n=18), Pseudomonas aeruginosa (n=10), Enterobacter cloacae (n=6), Acinetobacter baumannii (n=5), Klebsiella oxytoca (n=4), Proteus mirabilis (n=2) and Salmonella paratyphi (n=1). MIC testing showed that 58/89 (65 %) and 29/89 (32 %) isolates were resistant to meropenem and colistin, respectively, whereas 27/89 (30 %) isolates were resistant to both antibiotics. Escherichia coli, K. pneumoniae, K. oxytoca, Pseudomonas aeruginosa, and Enterobacter cloacae isolates were bla NDM-1-positive (n=20). Some strains of Escherichia coli, K. pneumoniae and K. oxytoca were bla OXA-181-positive (n=4). Class 1, 2 and 3 integrons were found in 24, 20 and 3 isolates, respectively. Nine NDM-1-positive Escherichia coli strains could transfer carbapenem resistance via plasmids to susceptible Escherichia coli AB1157. Meropenem and colistin showed synergy in 10/20 (50 %) isolates by 24 h time-kill studies.Conclusion. Our results highlight the distribution of carbapenem- and colistin-resistance in Gram-negative bacteria isolated from the Tamil Nadu region in South India.

KW - integron

KW - plasmid-bound resistance

KW - transferability

KW - New Delhi metallo-beta lactamases

KW - carbapenemases

U2 - 10.1099/jmm.0.000508

DO - 10.1099/jmm.0.000508

M3 - Article

VL - 66

SP - 874

EP - 883

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

ER -